微核形成与细胞周期关系的初步研究——Ⅳ.化学诱变剂诱发人淋巴细胞间期各阶段的微核形成  

Preliminary Studies of the Relationship Between Micronucleus Formation and Cell Cycle Ⅳ. Micronucleus Formation Induced by Chemical Mutagens at G., G_1, S and G_2 Phases of Human Lymphocytes

作　　者：薛开先[1] 马国建[1] 吴建中[1] 沈宗丽[1] 

机构地区：[1]江苏省肿瘤防治研究所,南京210009

出　　处：《Acta Genetica Sinica》1992年第1期17-21,共5页

摘　　要：本实验应用具有诱变作用的抗癌药:噻地哌、长春新碱,乙双吗啉等,体内或体外处理诱发人体外周血淋巴细胞微核,通过控制细胞培养时间,放射性自显影及中期细胞阻滞等方法,定量地分析了细胞间期各阶段的微核率(MNF)。本组实验结果表明,间期各阶段均可有不同程度的微核形成,其中最多的是G_1期,其次是G_2期和G_0期。S期细胞的MNF较G_1期有极显著的下降,这提示大部分G_1期的微核细胞不能进入S期,使细胞增殖中止,这可能是抗癌药物杀伤肿瘤细胞的机制之一。In this paper the authors studied quantitatively the mictonucleus formation at various phases of interphase in human lymphocytes indaced by chemical mutagens by means of control of cell culture intervals, autoradiography and block of metaphase cells etc. The results show that mutagenic anti-turner drugs: bimolane (treatment in vitro) and thio-tepa etc. (treatment in vivo) can induce micronucleus formation at various phases of interphase in lymphocytes. The frequency of micronucleus (MNF)induced at G1 phase is significantly higher than that at G0 and G?phase. The MNF at S phase of cells is obviously lower than at G1. This result suggests that most of the micronucleated cells att G1 phase do not enter S phase and micronucleus rarely form at S phase of cells.

关 键 词：微核形成 化学诱变剂 细胞周期 

分 类 号：Q343[生物学—遗传学]