玉米粗线期染色体的高分辨率染色粒图  

High Resolution Chromomere Map of Maize Pachytene Chromosomes

作　　者：沈大稜[1] 汪黔生 

机构地区：[1]复旦大学遗传研究所 [2]Department of Biological Sciences University of Maryland Baltimore COUnty Catonsville, Maryland 21228 USA

出　　处：《Acta Genetica Sinica》1992年第2期145-149,共5页

基　　金：美国NSF-DCB 8502732项目

摘　　要：经温和固定和展片的玉米小孢子母细胞减数分裂的粗线期染色体,在电镜下,着丝粒和大量的电子致密的染色粒清晰可辨。其数量和位置自减数分裂早偶线期至早双线期是稳定和可重复的。为此,我们对粗线期的10条双价体中各条分别进行详细的染色粒分析,构建了一个完整的染色体组的染色粒图。总共识别和定位了达430±12(根据7套染色体组计数平均)染色粒。染色粒图可用于确定原位杂交法定位基因的详细分布区域,染色体重排中断点的定位,识别外源单价体和由于不联会或脱联会而产生的单价体等。Meiotic chromosomes were prepared from mildly-fixed maize nucrosporocytcs. Well-spread chromosomes were observed in a transmission electron microscope. At the pachytene stage, the centromeres, knobs and numerous electron-dense chromomeres were clearly discerni-ble in the electron microscope. We observed that the chromomere pattern was stable and reproducible from the early zygotene stage to the early diplotene stage of meiosis. Therefore, we undertook detailed chromomere analysis on each of the 10 bivalents at the pachytene stage. A complete chromomere map of the maize genome was prepared. A total of 430 ± 12(N -= 7) chromomeres were recognized and mapped. The chromomere map can be used for regional assignment of genes in detailed in situ hybridization mapping, for localizing chromosomal break points involved in chromosomal rearrangement, for recognizing alien univalents and univalems resulting from asynapsis or desynapsis.

关 键 词：玉米 染色体 染色粒图 粗线期 

分 类 号：S513.032[农业科学—作物学]