鲑鱼生长激素cDNA的分子克隆和序列分析  

Cloning and Sequence Analysis of Salmon Growth Hormone cDNA

作　　者：宋诗铎[1] K.Y.Trinh 丘才良[2] 

机构地区：[1]天津医学院第二附属医院,300210 [2]加拿大多伦多大学临床生化和生化系

出　　处：《Acta Genetica Sinica》1992年第4期308-315,共8页

基　　金：加拿大医学研究委员会资助;多伦多儿童医院基金资助~~

摘　　要：从太平洋切奴克鲑鱼(Pacific Chinook Salmon,Oncorthychus tschawytscha)垂体poly(A)^+ RNA构建cDNA文库。按照鲑鱼生长激素(sGH)部分氨基酸序列合成两个寡聚脱氧核苷酸探针,它们分别与编码第1—7和第166—172氨基酸序列互补。用探针筛查cDNA文库,得到了完整的sGH cDNA克隆。cDNA序列已测定,包括编码210个氨基酸的编码序列。其中含有22个氨基酸的信号肽序列和188个氨基酸的成熟GH序列。该克隆还包括了5'端和3'端非翻译区,分别为72个和438个碱基对长。与Chum鲑鱼比较表明,核酸序列和氨基酸序列的同源性分别为97%和99%。cDNA clones encoding Chinook Salmon, Oncorhynchus tschawytscha, growth hormone (sGH) have been islated from a cDNA library prepared from Chinook Salmon pituitary gland pnly(A)+ RNA. Synthetic oligodeoxnucleotide mixtures based on amino acid residues 1-7 of sGH and 166-172 of sGH were used as hybridization probes to select recombinant plasmids carrying the sGH coding sequence. The complete nucleotide sequence of sGH cDNA has been determined. The cDNA sequence codes for a polypeptide of 210 amino acids including a putative signal sequence of 22 amino acids. The 5' and 3' untranslated regions of the message were 70 and 446 bases long, respectively. Comparison of the nucleotide sequence and amino acid sequence between Chum sGH and Chinook sGH indicated that there were 97% and 99% homology respectively.

关 键 词：CDNA文库 鲑鱼 生长激素 

分 类 号：Q959.403[生物学—动物学]