妊高征患者胎盘血管中PKC-α、NF-κB表达的研究  

Study on the expressions of protein kinase C-α,NF-κB in the plac enta vascula of PIH

作　　者：蒋荣珍[1] 陈汉平[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院妇产科,武汉430030

出　　处：《现代妇产科进展》2004年第1期16-18,共3页Progress in Obstetrics and Gynecology

摘　　要：目的 :探讨蛋白激酶C α(PKC α)、NF κB在妊高征 (PIH)胎盘绒毛血管病变中的作用。方法 :用免疫组化、图像分析等方法测定胎盘绒毛细小血管PKC α及NF κB的表达及对绒毛血管病变进行综合评价 ;用RT PCR测定胎盘组织前胶原Ⅰ、Ⅲ含量。结果 :(1 )PIH胎盘细小血管管壁增厚 ,管壁纤维素样坏死、急性粥样硬化发生率显著高于对照组 (P <0 .0 1 ) ;(2 )PIH胎盘绒毛细小动脉PKC α及NF κB显色强度明显高于对照组 ;(3)PIH胎盘绒毛组织前胶原Ⅰ明显高于对照组 (P <0 .0 1 ) ;(4)胎盘绒毛细小动脉管壁PKC α及NF κB的表达与细小动脉中膜平滑肌细胞核密度 (SMC)及胎盘绒毛组织前胶原Ⅰ的表达均呈正相关 (P <0 .0 5)。结论 :PKC α及NFObjective:To investigate the effects of protein kinase C-α (PKC-α) and nuclear factor kappa B(NF-κB) on the expression of placenta vascular collagen in pregnancy i nduced hypertention(PIH).Method:The PKC-α and NF-κB expre ssio ns in placental tissue were determined by immunohistochemistry,image analysis.Th e expressions of placental tissue precollagen Ⅰ?Ⅲ were determined by RT-PCR. Results:The thinkening,fibronoid necrosis,acute arterial ather osis of the placental vessel in PIH were significantly higher than those in normal g r oup.The collagenous fibers in placental arteris of PIH were higher than those in normal group.The expressions of PKC-α and NF-κB in placental vessel of PIH were greatly st rong er than those in normal group.The amount of placental vascular precollagen I in PIH was higher than those in normal group.Conclusion:The PKC -α?NF-κB signal pathway may regulate the expression of placenta and arteria l coll agen in PIH,which may play an important role in the pathogenesis of PIH.

关 键 词：妊娠并发症 胎盘 心血管 蛋白激酶C NF-ΚB 胶原 妊高征 

分 类 号：R714.246[医药卫生—妇产科学]