耳蜗神经传导及毛细血管张力中一氧化氮合酶的作用(英文)  

Role of nitric oxide synthase on nerve conduction and capillary blood flow in guinea pig cochlea

作　　者：潘世恺[1] 角南贵司子 山根英雄 王正敏[1] 

机构地区：[1]复旦大学医学院附属眼耳鼻喉科医院耳鼻喉科,上海市200031 [2]日本大阪市立大学医学部耳鼻咽喉科学教室

出　　处：《中国临床康复》2004年第4期782-783,F003,共3页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:探讨一氧化氮合酶在豚鼠耳蜗内的分布以及对毛细血管张力的作用机制。方法:经4g/L多聚甲醛心脏灌注固定后,取出耳蜗,经3g/L依地酸脱钙后,作10μm厚冰冻切片,用辅酶II孵育液在37℃条件下孵育1h。结果:发现在耳蜗内、外毛细胞底部与耳蜗神经末梢接头处及毛细血管球内皮细胞有明显NADPH-黄递酶活性,此外在内、外柱细胞、支持细胞、血管纹及螺旋神经节细胞也有NADPH-黄递酶活性反应。结论:一氧化氮(NO)在维持耳蜗正常神经传导及毛细血管张力中起着重要作用。AIM:To explore the effect and mechanism of nitric oxide synthase(NOS) in the regulation of nerve conduction and capillary blood flow in guinea pig cochlea. METHODS:After paraformaldehyde perfusion of the heart in 6 guinea pigs, the c ochleas taken to prepared frozen sections following decalcification with edetic acid.The sections were incubated in nicotinamide adenine dinucleotide phosphate hydrogen(NADPH) for charactering the distribution and activity of NADPH-diapho rase by means of enzyme-linked immunosorbent assay(ELISA). RESULTS:Obvious NAD PH-diaphorase activity was found in the guinea pig cochlea,cochlea nerve endin g underlying the outer hair cells,and the endothelial cells of the capillary glo mus. In addition,NADPH-diaphorase activity was also detected in the outer and inner pillar cells,supporting cells, spiral ligaments,and spiral ganglion cells. CONCLUSION:Nitric oxide is crucial in maintaining normal nerve conduction and capillary blood flow in the cochlea of guinea pigs.

关 键 词：耳蜗神经 神经传导 毛细血管张力 一氧化氮合酶 神经末梢 

分 类 号：R764.4[医药卫生—耳鼻咽喉科]