碱性成纤维细胞生长因子对大鼠骨骼肌拉伤修复的影响  

Effect of basic fibroblast growth factor on the healing of strain injured skeletal muscle in rats

作　　者：冯鑫[1] 王生[1] 李国珍[1] 

机构地区：[1]北京大学医学部公共卫生学院职业与环境卫生学系,北京市100083

出　　处：《中国临床康复》2004年第5期846-847,T001,共3页Chinese Journal of Clinical Rehabilitation

基　　金：国家自然科学基金资助项目(30171206)~~

摘　　要：目的:探讨外源性碱性成纤维细胞生长因子(bFGF)对大鼠骨骼肌拉伤后肌肉再生修复的影响。方法:雄性Sprague-Dawley大鼠,体质量约250g,随机区组法分为3组,每组6只:肌肉拉伤后给bFGF组、拉伤后给生理盐水对照组以及假拉伤组。用万能材料测试仪对大鼠腓肠肌造成拉伤后,于损伤肌肉皮下肌膜外注射bFGF(200AU/d×6)或生理盐水,免疫组织化学染色观察再生肌纤维中结蛋白的表达(用其积分光密度integraopticaldensity,IOD表示)。结果:生理盐水对照组结蛋白IOD值(25.79±10.44)×103显著高于假拉伤组(9.28±4.83)×103(t=13.85,P<0.01),bFGF治疗组肌肉结蛋白IOD值(34.48±10.62)×103高于生理盐水对照组(25.79±10.44)×103组,差异具有显著性(t=24.08,P<0.01)。结论:外源性碱性成纤维生长因子可以促进肌肉拉伤后的结蛋白表达,提高肌肉再生能力,从而改善肌肉损伤后的结构修复。AIM:To investigate the effect of exogenous basic fibroblast growth factor(bFGF) on the healing of strain injured skeletal muscles in rats. METHODS:Eighteen male Sprague Dawley rats were divided into three groups according to randomized block design:muscle strained bFGF treated group,muscle strained normal saline treated control group and sham strained group,each of which had 6 rats.The gastrocnemius muscles in the former two groups were strained caused by universal testing machine,and treated with bFGF(200 AU/d) and normal saline for six days,respectively.Desmin expression,an indicator of muscle regeneration(expressed as integra optical density,IOD) in muscle fibers, was measured by immunohistochemistry. RESULTS:The IOD of desmin was higher in the normal saline treated group[(25.79 ± 10.44) ×103]than the sham strained group[(9.28 ± 4.83) ×103](t=13.85,P < 0.01).The IOD of desmin in the bFGF treated group[(34.48 ± 10.62) ×103]was higher than the normal saline treated group[(25.79 ± 10.44) ×103](t=24.08,P< 0.01). CONCLUSION:Exogenous bFGF can improve desmin expression after the muscle is stained,and accelerate the regeneration of muscles,and thus facilitate the healing of muscle structure after muscle strain injury in rats.

关 键 词：碱性成纤维细胞生长因子 大鼠 骨骼肌拉伤 修复 肌肉再生 

分 类 号：R685[医药卫生—骨科学]