糖尿病高血脂所致大鼠肾组织SOD和GSH及MDA的变化  

Changes of SOD,GSH and MDA in renal tissues of diabetic hyperlipidemia rats

作　　者：曹和欣[1] 何立群[1] 沈雅静[1] 

机构地区：[1]上海中医药大学附属曙光医院肾内科,上海200021

出　　处：《中西医结合学报》2004年第1期36-38,共3页Journal of Chinese Integrative Medicine

基　　金：上海市卫生系统百人计划资助项目

摘　　要：目的 :通过比较两种糖尿病大鼠模型的代谢特点 ,研究大鼠肾组织超氧化物歧化酶 (superoxidedismutase,SOD) ,谷胱甘肽 (glutathione ,GSH) ,丙二醛 (malondialdehyde ,MDA)的变化。 方法 :采用高脂饲料联合小剂量注射链脲佐菌素 (streptozo tocin ,STZ) (模型 1组 )和多次小剂量注射STZ加福氏完全佐剂 (completeFreund’sadjuvant,CFA) (模型 2组 )复制大鼠糖尿病模型 ,测量大鼠体重、尿量、血糖、血脂、血清胰岛素 ,计算胰岛素敏感性指数 (insulinsensitivityindex ,ISI) ,并检测肾组织SOD、GSH、MDA的变化。结果 :两组大鼠的体重减轻 ,尿量增加 ,血糖明显升高 ,与正常组比较有显著差异 (P <0 .0 5 ) ;模型 1组大鼠的肾组织MDA升高 ,SOD、GSH较模型 2组降低更为明显 (P <0 .0 5 ) ;模型 2组大鼠的血清胰岛素水平下降 ,模型 1组血清胰岛素无明显下降 ,但ISI较模型 2组明显下降 (P <0 .0 5 )。结论 :糖尿病大鼠存在肾脏抗氧化酶的活性下降 。Objective:To study the changes of SOD, GSH and MDA in the renal tissues of diabetes rat models through the comparison of their metabolic features. Methods:Two types of diabetes rat models were established by feeding high-lipid food along with small dose injecting of streptozotocin (STZ) (model group 1) and repeated small dose injecting of STZ with complete Freund’s adjuvant (CFA) (model group 2). And changes of the following were observed: body weight, urine volume, blood sugar, blood lipid, serum insulin, insulin sensitive index(ISI),superoxide dismutase(SOD), glutathione(GSH) and malondialdehyde (MDA) of the renal tissues. Results:The body weight of the two model groups of rats decreased, while the urine volume and the blood sugar increased predominantly. These indexes of the two model groups were significantly different from those of the normal group (P <0.05). The renal tissue MDA level increased while the SOD and GSH level decreased in both model group 1 and model group 2,but the changes in model group 1 were more obvious than those in the model group 2. The serum insulin level which decreased in model group 2 did not decrease significantly in model group 1, but the ISI in model group 1 decreased more significantly than that in model group 2 (P <0.05). Conclusion:The antioxidase activity decreased in renal tissues of diabetes rats and hyperlipidemia was the main cause of the corresponding oxidations.

关 键 词：糖尿病 高血脂 大鼠 肾组织 SOD GSH MDA 谷胱甘肽 超氧化物歧化酶 

分 类 号：R587.2[医药卫生—内分泌]