核因子-κB/IκB信号通路介导实验性肾炎肾组织中单核细胞趋化蛋白-1表达  

Expression of monocyte chemoattractant protein-1 in experimental rat glomerulonephritis is mediated by NF-κB/IκB signal pathway

作　　者：张爱华[1] 黄松明[1] 丁桂霞[2] 吴元俊[3] 张维真[1] 吴红梅[1] 费莉[3] 郭梅[3] 陈荣华[3] 

机构地区：[1]南京医科大学附属南京儿童医院肾科,210008 [2]南京医科大学第二附属医院 [3]南京医科大学小儿肾脏病研究中心

出　　处：《中华病理学杂志》2003年第6期548-552,共5页Chinese Journal of Pathology

基　　金：国家自然科学基金资助项目 (3 0 10 0 0 81) ;江苏省教委自然科学基金资助项目 (99KJB3 2 0 0 0 3 )

摘　　要：目的　探讨核因子 κB(NF κB) /IκB信号通路在肾毒血清性肾炎肾组织单核细胞趋化蛋白 1(MCP 1)表达中的作用。方法　肾毒血清肾炎应用兔抗鼠肾小球基底膜肾毒血清制备。应用凝胶电泳迁移率 (EMSA)和Western印迹检测肾毒血清肾炎大鼠肾组织中NF κB活化、p6 5亚基核转位以及IκBα和IκBβ的降解 ;采用免疫组织化学及核酸酶保护法检测肾组织中MCP 1表达 ,并分析其与NF κB活化的关系。结果　模型组肾小球及肾小管中MCP 1表达分别为 (2 4 37± 7 0 6 )个 /肾小球横切面和 (5 4 78± 11 4 9) % ,较正常对照组显著升高 (P <0 0 1) ;肾组织中NF κB活化显著增强 ,p6 5由胞质转移至胞核 ,胞质内IκBα和IκBβ降解明显增加 ;NF κB活化与MCP 1表达呈显著正相关。 结论　NF κB/IκB信号通路介导肾小球肾炎肾组织中MCP 1表达。Objective To investigate the role of NF-κB/IκB signal pathway in mediating the expression of monocyte chemoattractant protein-1 (MCP-1) in experimental rat glomerulonephritis. Methods Nephrotoxic serum nephritis (NTN) was induced by injection of anti-GBM antibody into the tail veins of rats. Electrophoretic mobility shift assay (EMSA) and Western Blot were used to detect the activation of NF-κB, nuclear translocation of p65 subunit and degradation of IκBα and IκBβ in rat renal tissue. MCP-1 expression in glomeruli and renal tubules was also assessed by immunohistochemistry and ribonuclease protection assay. This was further correlated with the activation of NF-κB. Results There was an obvious expression of MCP-1 in glomeruli and renal tubules. Significant up-regulation of NF-κB activation, nuclear translocation of p65 subunit, and degradation of IκBα and IκBβ were also observed in NTN rat renal tissue, as compared to the control group. A positive correlation was noted between NF-κB activation and MCP-1 expression. Conclusions NF-κB/IκB signal pathway may play an important pathogenetic role in glomerulonephritis, with mediating the expression of MCP-1.

关 键 词：NF-κB 信号通路 肾炎 单核细胞趋化蛋白-1 炎症介质 

分 类 号：R692.3[医药卫生—泌尿科学]