增生性瘢痕内p38丝裂素活化蛋白激酶及其MAPKKs基因的表达  被引量:3

GENE EXPRESSION OF p38 MITOGEN-ACTIVATED PROTEIN KINASE AND ITS MAPKKs IN HYPERTROPHIC SCARS

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作  者:陈伟[1] 付小兵[1] 孙晓庆[1] 赵志力[1] 周岗[1] 孙同柱[1] 盛志勇[1] 

机构地区:[1]解放军第304医院全军烧伤研究所,北京100037

出  处:《解放军医学杂志》2003年第7期592-593,共2页Medical Journal of Chinese People's Liberation Army

基  金:国家重点基础研究规划 (编号G1 9990 542 0 4 );国家自然科学重点项目(编号 30 2 30 370 );国家自然科学基金 (编号 30 1 70 966)资助课题

摘  要:为探讨增生性瘢痕和正常皮肤中p3 8丝裂素活化蛋白激酶 ( p3 8MAPK)及其上游信号分子mkk3和mkk6基因表达的变化 ,提取 8例增生性瘢痕和 8例正常皮肤组织的总RNA后 ,分离纯化mRNA ,用RT PCR方法检测这 3种基因在不同组织中的表达变化规律。结果显示 ,在正常皮肤组织中 ,mkk6和p3 8MAPK基因表达较弱 ,而在增生性瘢痕内 ,这 2种基因PCR产物的灰度比分别为正常皮肤的 1 2倍和 1 9倍 ,基因表达量明显升高 (P <0 0 5和P <0 0 1) ,而mkk3在这两种不同类型组织中的表达量没有显著性差异 (P >0 0 5 )。提示增生性瘢痕的发生可能与mkk6和 p3 8MAPK基因表达升高有关 。To explore the change in gene expressions of p38 mitogen-activated protein kinase (p38MAPK) and its upstream signal-regulated molecule (mkk3 and mkk6) in normal skin versus hypertrophic scars underlying its potentially biological significance. Total RNAs were isolated from 8 specimens of hypertrophic scars and 8 specimens of normal skin, then they were purified to mRNAs, and the gene expression of mkk3, mkk6 and p38MAPK was examined with reverse transcription-polymerase chain reaction (RT-PCR). The results showed that the intensities of gene expression of mkk6 and p38MAPK were weak in normal skins, while the expression of these 2 genes was significantly elevated in hypertrophic scars compared with normal skins (P<0.05 and P<0.01). The expression intensities of these 2 genes were 1.2 folds and 1.9 folds of normal skins, respectively. Mkk3 gene expression showed no substantial change in normal skins compared with hypertrophic scars (P>0.05). In hypertrophic scar, the elevation of mkk6 and p38MAPK gene expression, which plays pilot roles in cell proliferation, may be one of the mechanisms controlling the formation of hypertrophic scars.

关 键 词:P38MAP激酶 mkk3基因 mkk6基因 瘢痕 

分 类 号:R619.6[医药卫生—外科学]

 

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