幼鼠梭箱回避学习行为与脑内相关递质系统发育的关系  

Relationship between the behavior of shuttle-box avoidance conditioning and the development of brain transmitter system in infant rats

作　　者：嵇志红[1] 于新宇[2] 王辉[3] 

机构地区：[1]大连大学医学院生理教研室,辽宁省大连市116622 [2]大连大学医学院组胚教研室,辽宁省大连市116622 [3]大连大学医学院机能实验室,辽宁省大连市116622

出　　处：《中国临床康复》2004年第7期1238-1239,共2页Chinese Journal of Clinical Rehabilitation

基　　金：辽宁省教委基金资助课题(9606311126)~~

摘　　要：目的:探讨幼鼠脑内胆碱能和肾上腺素能递质系统的发育与梭箱回避学习行为(shuttle-boxavoidanceconditioning,SAC)的关系。方法:以8～25日龄幼年SD大鼠为实验对象,随机将其分为8,17,21,25日龄4大组,每大组又分为东莨菪碱组(A组)、酚妥拉明组(B组)、生理盐水对照组(C组)3组。8日龄组每日分别腹腔注射东莨菪碱(3mg/kg)、酚妥拉明(5mg/kg)和生理盐水,连续注射10d后,进行SAC实验。另外3组腹腔注射上3种药15min后即开始SAC实验。分别观察各组幼鼠SAC行为的正确反应次数和反应潜伏期。结果:与生理盐水对照组比,东莨菪碱对17～21日龄幼鼠的SAC学习行为无明显影响,对21日龄以后幼鼠逐渐表现抑制效应,表现为SAC的正确反应次数明显降低犤(18.7±6.4)次比(8.4±5.3)次,t=3.916,P<0.01犦,反应潜伏期明显升高犤(1.0±0.6)次比(60.9±10.3)次,t=18.348,P<0.01犦;酚妥拉明对8～25日龄幼鼠的SAC学习行为均呈抑制效应。结论:幼鼠梭箱回避学习行为与脑内不同递质系统的发育有密切关系。AIM:To study the relationship between the behavior of shuttle-box avoidance conditioning(SAC) and the development of cholinergic and adrenergic transmitter systems. METHODS:Sprague-Dawley infant rats of 8-25 days were randomly divided into 4 groups of 8 days,17 days,21 days and 25 days.Each group was subdivided into 3 groups again:scopolamine group(A),phentolamine group(B) and saline control group(C).Eight-day rats received daily intraperitoneal injection of scopolamine(3 mg/kg),phentolamine(5 mg/kg) and saline respectively for 10 days.The SAC test was performed after 10 days.The other three groups began SAC test 15 minutes after receiving scopolamine, phentolamine and saline through intraperitoneal injection.The times of correct reaction and the latency of SAC were observed. RESULTS:Compared with the saline group,scopolamine could inhibit the behavior of SAC in the rats with the age of 21 days,exhibiting a decrease in the reaction times[(18.7±6.4) times vs(8.4±5.3) times](t=3.916, P < 0.01),and an increase in the latency [(1.0±0.6) s vs(60.9±10.3) s](t=1.348,P< 0.01),but had no obvious influence in the rats of 17-21 days;Phentolamine could inhibit the behavior of SAC at the age of 8-25 days. CONCLUSION:The behavior of SAC in the infant rats is correlated with the development of different transmitter systems in the brain.

关 键 词：幼鼠 回避学习 胆碱能 肾上腺素能 神经递质 东莨菪碱 酚妥拉明 生理盐水 

分 类 号：R338[医药卫生—人体生理学] R-33[医药卫生—基础医学]