大鼠胎脑神经细胞与几丁质多孔体、胶原蛋白海绵及明胶海绵生物相容性的研究(英文)  

Study on the biocompatibility of rat fetal neurons with chitin,collagen and glutin sponge

作　　者：田波[1] 刘慧雯[1] 吴树亮[2] 于宏伟[1] 方冬云[3] 

机构地区：[1]哈尔滨医科大学组织胚胎学教研室,黑龙江省哈尔滨市150086 [2]哈尔滨医科大学神经生物学教研室,黑龙江省哈尔滨市150086 [3]哈尔滨医科大学整形外科,黑龙江省哈尔滨市150086

出　　处：《中国临床康复》2004年第7期1398-1400,F003,共4页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:评价大鼠胎脑神经细胞与几丁质多孔体、胶原蛋白海绵及明胶海绵的细胞相容性,为中枢神经组织工程材料的选择提供实验依据。方法:将大鼠胎脑神经细胞分别与几丁质多孔体、胶原蛋白海绵及明胶海绵联合体外培养,进行光、电镜观察,并测定3种材料与细胞的吸附率。结果:神经细胞均能在3种材料上贴附并生长,细胞吸附率分别为31.54%,30.58%和15.54%,并且几丁质有促进细胞生长的作用。结论:几丁质、胶原蛋白及明胶3种材料,尤其是几丁质,具有良好的神经细胞相容性,有可能作为神经系统组织工程的载体应用于临床。AIM:To evaluate the cellular compatibility of rat fetal neuron with chitin polyporous carrier,collagen and glutin sponge,and to provide experimental evidence for material selection in central nervous tissue engineering.METHODS:Rats' neurons were co-cultured with chitin multiporous carrier,collagen and glutin sponge in vitro.Light microscope and electronic microscope morphymetry were utilized and the attachment rate of those three different materials were determined.RESULTS:Neurons could attach well to all these three materials, their attachment rates being 31.54%, 30.58%and 15.54%,respectively.Moreover,cell growth could be greatly facilitated by chitin.CONCLUSION:Materials made of chitin,collagen and glutin, especially chitin,show a fairly good biocompatibility and are promising nervous system tissue engineering materials for further clinical application.

关 键 词：大鼠 神经细胞 几丁质多孔体 胶原蛋白海绵 明胶海绵 生物相容性 生物医学工程 

分 类 号：R318[医药卫生—生物医学工程] R-33[医药卫生—基础医学]