rCHO细胞无血清适应及悬浮培养  

Adaption and Suspension Culture of rCHO Cells Producing HBsAg in Serum-free Medium

作　　者：黄斌[1] 牛红星[1] 朱明龙[1] 金秀杰 谭文松[1] 戴干策[3] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237 [2]华北制药集团新药研究所,石家庄050015 [3]华东理工大学联合化学反应工程研究所,上海200237

出　　处：《华东理工大学学报（自然科学版）》2004年第1期38-42,共5页Journal of East China University of Science and Technology

基　　金：上海市启明星计划(98QF1403);上海市高等学校青年科学基金的资助

摘　　要：考察了血清对rCHO(C28)细胞生长与产物(HBsAg)表达的影响,发现φ血清<0.01时细胞出现明显的代谢转换;考察了微量元素、氢化可的松、混合脂类与短多肽混合物(TL混合物)对细胞的影响,建立了适于rCHO(C28株)生长的无血清培养基MT-SFM;在将细胞从有血清转到无血清状态时,阶段性降血清比直接降血清更有利于rCHO(C28)细胞的无血清适应;当细胞适应了MT-SFM无血清培养基后,批培养中细胞的最大平均比生长速率可达0.65d-1,产物HBsAg的滴度在32～64之间。Effects of different concentration of serum on the growth and HBsAg production of rCHO(C28) cells were studied, and the critical concentration of serum for the metabolic change of rCHO (C28) cells determined was φ__(NBS)<0.01. Effects of trace elements, hydrocortisone, lipid mixture and TL mixture on the growth of rCHO (C28) cells were also studied, and MT-SFM, a new serum-free media suited for rCHO (C28) cells, has been successfully developed. When rCHO (C28) cells were transferred from media with serum to serum-free media, the gradual adaptation was found to be more benificial than the direct adaptation. After rCHO (C28) cells have adapted to MT-SFM, the maximum of average specific growth rate was 0.65 d^(-1) and the titre of HBsAg was between 32 and 64 in batch culture.

关 键 词：RCHO细胞 无血清培养基 无血清适应 乙肝表面抗原 HBSAG 悬浮培养 

分 类 号：Q813.1[生物学—生物工程]