恙虫病立克次体DNA及DNA序列测定  

DETERMINATIONSOF DNA AND DNA SEQUENCESOF RICKETTSIA ORIENTALIS

作　　者：詹志农 [1] 王华民 [1] 陶启健  Yu Xue-jie  林碧瑚 [1] 

机构地区：[1]海南医学院病原生物学与免疫检测实验室,海口,571101 [2]Department of Pathology,University of Texas Medical Branch (UTMB),Galveston of Texas TX77555-0609,USA [3]海南医学院微生物学教研室,海口,571101

出　　处：《海南医学院学报》2004年第1期18-20,共3页Journal of Hainan Medical University

摘　　要：目的:检测39例不明原因发热患者血液中恙虫病立克次体DNA。方法:用NPCR检测恙虫病立克次体DNA,取其DNA提取物进行DNA序列分析。结果:序列分析表明扩增的片段为立克次体DNA片段。39例不明原因发热患者血液用NPCR法检出13例恙虫病立克次体DNA。占不明原因发热患者的33.3%。结论:NPCR法可以快速检出恙虫病立克次体DNA,可用于恙虫病的临床早期诊断。Objective :To detect the Rickecttsia tsutsugamushi DNA from blood specimens of39cases of patients with fever unknown origin ( FUO ) .Method :DNA of Rickecttsia tsutsugamushi was detected by using NPCR and the specific isolation products from the DNA were used for DNA cloning and the DNA sequence analysis.Res ults :DNA sequence analysis confirmed the sequence was the fragment of Rickecttsia tsutsugamushi DNA.13cases of Rickecttsia tsutsugamushi DNA were de鄄tected from all39blood specimens of FUO patients ( 33.33% ) .Conclus ion :It is possible to diagnose clinically the scrub fever early and quickly with NPCR.

关 键 词：恙虫病立克次体 DNA序列 NPCR法 发热 早期诊断 

分 类 号：R513.2[医药卫生—内科学]