白桦雄花序发育初期蛋白质的双向电泳图谱分析  

Anatomical Observation and Image Analysis of Two-dimensional Gel Electrophoresis of Male Flower in the Early Period of Inflorescene Development for Betula platyphylla Suk

作　　者：杨传平[1] 姜静[1] 梁艳[1] 刘桂丰[1] 王大海[1] 李慧玉[1] 

机构地区：[1]东北林业大学,哈尔滨150040

出　　处：《东北林业大学学报》2004年第1期1-4,共4页Journal of Northeast Forestry University

基　　金：国家科技攻关课题资助 (课题编号 0 0 2BA51 5B0 5)

摘　　要：对发育初期 2个时期的白桦雄花序进行的蛋白质双向电泳 (two -dimensionalelectrophoresis,2 -DE)研究结果表明 ,2个时期的 2 -DE图谱 ,有 2 75个蛋白点在表达上有明显的质和量的变化。其中 :16 2个蛋白点在 5月 31日雄花序 2 -DE图谱中的表达量均高于 5月 2 8日 ,110个蛋白点的表达量低于 5月 2 8日 ;2个蛋白点为 5月 2 8日雄花序 2 -DE图谱所特有 ;1个蛋白点为 5月 31日雄花序 2 -DE图谱所特有。这些结果为进一步获得与雄花序发育时期的相关蛋白 ,进而得到相关的特异基因 ,在分子水平上揭示白桦雄花序发育机理奠定重要基础。Two-dimensional electrophoresis (2-DE) patterns of male inflorescence for Betula platyphylla were studied. The result showed that there existed 275 differential protein spots in quality and quantity on two 2-DE maps of male inflorescence at two stages in the early period of development, of which 162 spots increased and 110 spots decreased in quantity, 2 spots were missing and 1 new spot emerged in male inflorescence on May 31st compared with that on May 28th. The results can lay down an important foundation in obtaining correlative protein and differential gene in specific period of inflorescence development as well as the development mechanism of male inflorescence in molecular level in Betula platyphylla.

关 键 词：白桦 雄花序 发育初期 蛋白质双向电泳 电泳图谱 

分 类 号：S792.153[农业科学—林木遗传育种]