雌雄文昌鱼同工酶的表型差异  

Sex-related differences in isozymic phenotypes in amphioxus Branchiostoma belcheri tsingtauense

作　　者：庞秋香[1] 张士璀[1] 王长法[1] 王昌留[1] 张永忠[1] 

机构地区：[1]中国海洋大学海洋生物系,青岛266003

出　　处：《动物学报》2004年第1期62-67,共6页ACTA ZOOLOGICA SINICA

基　　金：国家自然科学基金资助项目(No .39770 0 91 )~~

摘　　要：本文应用聚丙烯酰胺凝胶电泳结合生化染色方法分析了雌雄文昌鱼中苹果酸酶、苹果酸脱氢酶、酸性磷酸酶和酯酶四种同工酶的酶谱。首次发现苹果酸酶、苹果酸脱氢酶和酸性磷酸酶表型在文昌鱼雌性和雄性个体之间存在差异 ,而在同一性别不同个体之间无差异。酯酶表型较复杂 。Four isozymes, i.e. malic enzyme, malate dehydrogenase, acid phosphata se and esterase, in amphioxus Branchiostoma belcheri tsingtauense, were anal yzed using polyacrylamide gel electrophoresis (PAGE) and biochemical staining. It wa s found that (1) the electrophoretic patterns of the isozymes showed sex-relate d differences between male and female amphioxus; (2) the electrophoretic patterns of malic enzyme, malate dehydrogenase and acid phosphatase did not show inter- i ndividual difference in the same sex; (3) however, the electrophoretic patterns of esterase showed some differences in both sexes as well as in different indivi duals of the same sex .

关 键 词：文昌鱼 苹果酸酶 苹果酸脱氢酶 酸性磷酸酶 酯酶 

分 类 号：Q55[生物学—生物化学] Q959.4