中国石龙子雄性生殖腺的年周期变化  

Annual variation in gonads of male Chinese skinks Eumeces chinensis

作　　者：胡健饶[1] 杜继曾[1] 计翔[2] 

机构地区：[1]浙江大学生命科学学院,杭州310027 [2]杭州师范学院生命科学学院,杭州310036

出　　处：《动物学报》2004年第1期103-110,共8页ACTA ZOOLOGICA SINICA

基　　金：浙江省动物学重点扶植学科资助项目~~

摘　　要：20 0 1年 3月至 2 0 0 2年 2月期间 ,通过每月捕捉浙江丽水中国石龙子 (Eumeceschinensis)雄性成体 ,解剖动物、观测性腺的形态和组织学特征 ,研究雄性生殖周期。睾丸重量和体积、附睾重、输精管重和曲细精管直径有显著的季节变化。睾丸 3月份最重 ,5-9月最轻。睾丸体积和重量的年周期变化规律一致。附睾 3月份最重 ,8-9月份最轻。输精管 4月最重 ,8-10月最轻。生精活动始于 9月下旬 ,翌年 4月最为活跃。 3月下旬曲细精管直径达全年最大值 ,管腔中开始出现呈穗状排列的精子。从基膜到管腔 ,各级生精细胞依次排列。 4月份生精上皮的生精活动最为活跃 ,5月下旬生精活动已近停止 ,7-8月份曲细精管管壁仅由精原细胞 (其间夹有支持细胞 )构成。根据曲细精管生精上皮的年周期变化规律 ,中国石龙子 8月份睾丸生精活动处于第Ⅰ期 ,9月至次年 2月份第Ⅱ期 ,3月上、中旬为Ⅲ期 ,3月底至 4月为Ⅳ期 ,5-6月份为Ⅴ期 ,7月份为Ⅵ期。 4月下旬附睾管腔中有大量的成熟精子 ,7月附睾管腔中已无精子。From March 2001 to February 2002, we collected monthly 8-15 adult males of E. chinensis from a population in Lishui, Zhejiang, eastern China, to s tudy the male reproductive cycle. The captured skinks were transported to our la boratory at Hangzhou Normal College, where they were measured, weighed, and diss ected. Testes, epididymides, and ductus deferens were removed from each dissecte d skink, weighed, and fixed with Bouin’s solution for 24 h. The fixed tes tes w ere then measured for length (2a) and width (2b), which were used in the equatio n of ellipsoid [V= (4/3) π ab 2] for calculating the volume of testis. Paraffin s lices were prepared for testes, epididymides, and ductus deferens following rout ine procedures, so that the structural and morphological features of these compo nents could be observed. Seasonal changes in testes, epididymides, and ductus de ferens were very pronounced in E. chinensis. The largest testes were observe d in March, and the smallest from May to September. Epididymides were also largest i n March, but smallest from August to September. Ductus defference were largest i n April, and smallest from August to October. A rapid increase in the thickness of the germinal epithelium occurred in late September, suggesting an initiation of testicular recrudescence. After the winter dormancy, the diameter of seminife rous tubules increased steadily and reached its peak in late March, when sperms had already appeared inside the lumen of seminiferous tubules. Spermatogenesis w as very active in April, but ceased soon in late May. A larger number of matured sperms could be found in the lumen of ductus epididymis in late April, but none could be found in July. The wall of the seminiferous tubules consisted of almos t a single layer of spermatogonia and Sertoli cells in July and August. Based on descriptions by Radder et al. (2001) for the annual cycle of testicular activit y of lizards, annual testicular activities of E. chinensis can be divided in to s ix stages, including StageⅠin Augus

关 键 词：中国石龙子 雄性生殖周期 睾丸 附睾 输精管 曲细精管 石龙子科 蜥蜴 

分 类 号：Q492[生物学—生理学]