河南花背蟾蜍的核型、C-带和Ag-NORs研究  

Karyotype,C-banding and Ag-NORs of Bufo raddei from Henan

作　　者：董自梅[1] 周洁 常重杰[3] 

机构地区：[1]濮阳职业技术学院,濮阳457000 [2]平顶山市二十七中学,平顶山467000 [3]河南师范大学生命科学学院,新乡453002

出　　处：《动物学杂志》2004年第1期29-35,共7页Chinese Journal of Zoology

摘　　要：利用骨髓细胞蒸汽固定法制备染色体标本 ,研究了分布于河南新乡的花背蟾蜍 (Buforaddei)的核型、C 带和Ag NORs。结果表明 :河南产花背蟾蜍体细胞染色体数为 2 2条 ,核型公式为 2n =2 2 ( 2 0m +2sm) ,全部为中部或亚中部着丝点染色体 ,NF =4 4。Ag NORs具有多态现象。C 带核型显示 2 2条染色体的着丝点均正染 ,No .1染色体近着丝粒处有不恒定插入型C 带 ,No.4染色体具有恒定近端部插入型C 带 ,随体部位被正染的仅占所观察细胞的 15%。The chromosome samples were prepared from metaphase marrow cells from Bufo rad dei which distributed in Xinxiang, Henan by steam-fixation method. Karyotype, C-banding and silver-staining nucleolus organizer regions (Ag-NORs) were obse rved on chromosomes. The chromosome number of Bufo raddei is 2n=22(20m+2sm), and all of the chromosomes were metacentric or submetacentric (NF=44). In additi on, polymorphism was found in Ag-NORs whereas all the chromosomes were stained in C-banding. There was a variable insert type C-banding near centromere in No .1 chromosome and an invariable insert type C-banding near terminal position in No.4 chromosome.

关 键 词：核型 C-带 AG-NORS 花背蟾蜍 河南 骨髓细胞蒸汽固定法 染色体 

分 类 号：Q953[生物学—动物学] Q959.53