RNA干涉在纤毛虫中的研究进展  

Application of RNA Interference in the Study of Ciliated Protozoa

作　　者：刘星吟[1] 黄炫[1] 樊彧[1] 金立培[1] 

机构地区：[1]中山大学生命科学学院,广州510275

出　　处：《动物学杂志》2004年第1期117-121,共5页Chinese Journal of Zoology

基　　金：国家自然科学基金资助项目(No.3 9970 0 89)

摘　　要：RNA干涉是dsRNA介导的基因沉默现象 ,本文简要介绍了其作用的机制和生物学意义 ,重点阐述了RNA干涉在原生动物纤毛虫中的发现与应用 ,比较了RNA干涉与纤毛虫大核基因组重排机理的异同 ,并对RNA干涉在纤毛虫中传输的技术途径RNA interference(RNAi)is the process of sequence-specific, post-tra nscriptional gene silencing by double-stranded RNA(dsRNA)that is homologous i n sequence to the silenced gene. RNA interference has been proved to be a powerfu l method for reducing the expression of specific genes in many organisms. Recent researches have shown that small RNAs function to specify sequences to be elimi n ated by a mechanism similar to RNA-mediated gene silencing during the gene rear r angement of the ciliated protozoa, Tetrahymena. The review will focus on the practical application of RNAi,the RNAi pathway in the ciliated protozoa.

关 键 词：纤毛虫 双链RNA RNA干涉 基因沉默现象 生物学 RNAi喂饲法 

分 类 号：Q953[生物学—动物学]