海藻酸诱导复杂部分发作癫痫大鼠海马GABA_A受体α_1亚单位的表达  

GABA_ARα_1 subunit expression in KA induced CPS rats

作　　者：雷革非[1] 王纪文[1] 孙若鹏[1] 王艳玲[1] 李保敏[1] 胡瑞梅[1] 

机构地区：[1]山东大学齐鲁医院儿内科,山东济南250000

出　　处：《山东大学学报（医学版）》2004年第1期67-70,共4页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金资助项目(Y2001C17)

摘　　要：目的:研究海藻酸(KA)致痫大鼠海马GABAA受体α1亚单位的动态表达水平以及加巴喷丁(GBP)干预对它们的影响,探讨复杂部分发作癫痫(CPS)的发生机制,为开发针对GABA受体亚单位水平的新型抗癫痫药物(AEDs)提供理论依据。方法:成年雄性Wistar大鼠海马CA3区注射2μgKA建立复杂部分发作模型,采用免疫组化SABC法检测癫痫大鼠海马各区GABAA受体α1亚单位(GABAARα1)的动态表达水平,并用GBP干预癫痫的发作观察GBP对GABAARα1表达的影响。结果:KA致痫后海马CA1区、CA3区GABAARα1表达呈先升后降趋势,齿状回(DG)表达水平则逐渐增加。GBP对癫痫早期GABAARα1表达无直接影响。结论:在KA诱导的CPS模型中,GABAARα1表达减少和由此所引起的抑制功能减弱,可能是癫痫发生的一种分子机制。Objective: To study the time-dependent expressions of GABAA receptorα1 subunit in KA induced complex partial seizure(CPS) rats, and the interference of gabapentin(GBP) on GABAA receptorα1 subunit. Methods: An epileptic model in male Wistar rats was developed by intracerebral injection of KA into CA3 subfield in hippocampus. The expressions of GABAA receptorα1 subunit on hippocampus were assayed by immunohistochemistry method. Results: The GABAA receptorα1 subunit expression levels in hippocampus(CA3?CA1 and dentate gyrus) of KA-induced rats within 24 hours were significantly elevated in contrast with that of contral group (P<0.01). Then the expression levels decreased in CA3?CA1 regions, and increased in dentate gyrus. Conclusion: In KA induced epilepsy model,the weakening of inhibition in hippocampus by the decreasing expression levels of GABAA receptorα1 subunit, may be a molecular mechanism of CPS.

关 键 词：癫痫 复杂部分性 受体 GABA—A 海藻酸 

分 类 号：Q786[生物学—分子生物学]