高效液相色谱法测定鸡蛋中氯霉素残留量  

Measurement on Chloramphenicol Residues in Eggs by HPLC

作　　者：岳秀英[1] 彭莉[1] 高岚[1] 

机构地区：[1]四川省兽药监察所,四川成都610041

出　　处：《四川畜牧兽医》2004年第3期22-23,共2页Sichuan Animal & Veterinary Sciences

摘　　要：采用高效液相色谱法(HPLC)检测鸡蛋中氯霉素的残留量。鸡蛋(全蛋)试样中残留的氯霉素用乙酸乙酯提取,35℃减压蒸干。残渣用正己烷-氯仿(1+1,V/V)溶解,加少量水,混匀后,离心,取上清液作为试样溶液供高效液相色谱仪(紫外检测器)在280nm处测定,外标法定量。本方法在鸡蛋中检出限为2μg/kg,回收率在60％～90％,批内变异系数小于12.0％,批间变异系数小于15.0％。HPLC method was used to isolate and measure the chloramphenicol residues in eggs.Samples with ehloramphenicol in eggs were extracted by ethyl acetate.and the extracts were purified with a mixture of chloroform-hexane(1+ 1.V/V),chloramphenicol was quantified by high performance liquid chromatography(HPLC) on silica coated column type C18 with UV detection at 280nm.and with the mobile phase of acetonitrile-water (20+80,V/V).The results showed that the average recovery rate was 60%-90%.the average coefficient of variation was less than 15.0%.

关 键 词：鸡蛋 氯霉素 药物残留量 高效液相色谱法 

分 类 号：S859.84[农业科学—临床兽医学] R155.56[农业科学—兽医学]