模拟微重力条件下新生大鼠心肌细胞三维培养体系的构建  

PILOT STUDY OF NEONATAL RAT CARDIAC MYOCYTES CULTURED FOR THREE-DIMENSIONAL MODELING IN SIMULATED MICROGRAVITY

作　　者：杨芬[1] 李莹辉[1] 聂捷琳[1] 毕蕾[1] 黄增明[1] 谭映军[1] 熊江辉[1] 

机构地区：[1]航天医学工程研究所航天细胞分子生物学实验室,北京100094

出　　处：《中国修复重建外科杂志》2004年第2期119-122,共4页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：国家高技术研究发展计划 (863)资助项目 (9632 2 1 0 3)

摘　　要：目的　研究模拟微重力条件下构建新生大鼠心肌细胞三维培养体系的方法。　方法　分离新生大鼠原代心肌细胞 ,接种于聚乳酸 (PL A)支架材料上 ,经搅拌瓶培养 2 4小时后转入旋转式细胞培养系统 ,用倒置相差显微镜、扫描电镜和透射电镜观察心肌细胞在支架上的生长情况 ,以四甲基偶氮唑盐 (MTT)比色法检测细胞活性。　结果　心肌细胞在 PL A支架材料上贴壁、伸展 ,融合成片 ,保持持续的代谢活性 ,发现 PL A-心肌细胞复合构造物的搏动。结论　接种于 PL A支架材料上的原代心肌细胞 ,在旋转式细胞培养系统的模拟微重力条件下 ,可以进行较理想的三维培养。Objective To study three-dimensional culturing methods of neonatal rat cardiac myocytes in simulated microgravity. Methods Neonatal rat primary cardiac myocytes were separated and seeded into polylactic acid scaffolds, stirred in spinner flasks for 24 hours, and then moved into rotary cell culture system for three-dimensional culture. The growth of cardiac myocytes was observed under inverted phase contrast microscope, scanning electron microscope and transmission electron microscope, and metabolic assay was assessed by MTT assay. Results Cardiac myocytes with sustained metabolic activity attached to the polylactic acid scaffolds, extended and confluenced. Pulsations of PLA-cardiac myocytes was found in some areas. Conclusion The rotary cell culture system is suitable to develop neonatal rat cardiac myocytes culturing for three-dimensional modeling.

关 键 词：微重力模拟 新生大鼠 心肌细胞 三维培养 心脏疾病 高纵横比容器 聚乳酸 

分 类 号：R85[医药卫生—航空、航天与航海医学]