Determination of cell volume during equilibrium freezing process  被引量:4

Determination of cell volume during equilibrium freezing process

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作  者:ZHAOGang HELiqun WANGPeitao DINGWeiping XIEXiaojian LIUZhong ZHANGHaifeng SHUZhiquan LUODawei GAODayong 

机构地区:[1]CryobiomedicalEngineeringResearchInstitute,DepamnentofThermalScienceandEnergyEngineering,UniversityofScienceandTechnologyofChina,Hefei230027,China [2]SchoolofLifeSciences,UniversityofScienceandTechnologyofChina,Hefei230027,China [3]RedCrossandBloodCenterofHefeiHefei230022,China [4]DepartmentofMechanicalEngineering,UniversityofKentucky-Lexington,KY40506-0108,USA

出  处:《Chinese Science Bulletin》2003年第15期1551-1554,共4页

基  金:supported by the Chang-Jiang Scholar Award,Hundred-Talent Program of the Chinese Academy of Sciences(2000-2003);the National Natural Science Foundation of China(Grant No.50106016);the Provincial Natural Science Foundation of Anhui Province(Grant Nos.00047520 and 03043717).

摘  要:A new type electronic particle counter (EPC, MultisizerTM 3, Beckman Coulter Inc., USA) was used to determine the volumes of human red blood cells (RBCs) in NaCl solutions of different osmolalities. The thermodynamics model describing cell response during freezing process was used to simulate the volume change of RBC in 0.9% NaCl solution during equilibrium freezing process. It was assumed that the effect of temperature on cell volume can be neglected compared to that of osmolality, then by using the phase diagram for the binary system sodium chloride/water, the osmolalities of the NaCl solution under different sub-zero temperatures can be obtained (converted from mass concentration), then the calculated values of RBC volumes can be validated by the experiments.A new type electronic particle counter (EPC, MultisizerTM 3, Beckman Coulter Inc., USA) was used to determine the volumes of human red blood cells (RBCs) in NaCl solutions of different osmolalities. The thermodynamics model describing cell response during freezing process was used to simulate the volume change of RBC in 0.9% NaCl solution during equilibrium freezing process. It was assumed that the effect of temperature on cell volume can be neglected compared to that of osmolality, then by using the phase diagram for the binary system sodium chloride/water, the osmolalities of the NaCl solution under different sub-zero temperatures can be obtained (converted from mass concentration), then the calculated values of RBC volumes can be validated by the experiments.

关 键 词:细胞容积 平衡凝固 电子粒子计数器 EPC 红血细胞 

分 类 号:TH724[机械工程—仪器科学与技术]

 

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