5α-还原酶在良性增生前列腺组织中的表达及意义  

Expression and significance of steroid 5α-reductase isoenzymes in benign hyperplastic prostate tissues

作　　者：叶烈夫[1] 张元芳[2] 方祖军[2] 丁强[2] 姚孟树[2] 

机构地区：[1]福建省立医院泌尿外科,福州350001 [2]复旦大学华山医院泌尿外科

出　　处：《中华医学杂志》2003年第15期1296-1299,共4页National Medical Journal of China

基　　金：福建省科技基金资助项目 (2 0 0 0E12 2 )

摘　　要：目的　了解Ⅰ型与Ⅱ型 5α 还原酶在良性增生前列腺组织中的表达情况 ,为前列腺增生的药物治疗提供理论依据。方法　用免疫组织化学及RT PCR方法对 15例正常前列腺组织和 2 5例良性增生前列腺组织中的两型 5α 还原酶同工酶的表达进行定性及半定量分析。结果　良性前列腺增生组织及正常前列腺组织中均存在两型 5α 还原酶的表达 ,阳性染色主要位于腺上皮细胞的胞质内。增生前列腺组织中Ⅱ型 5α 还原酶mRNA的表达水平明显高于正常前列腺组织 (分别为 1 5 0±0 4 2及 0 98± 0 32 ,P <0 0 1) ,而Ⅰ型还原酶的表达相对较弱且与正常前列腺组织比较差异无显著意义 (分别为 0 6 2± 0 31与 0 5 1± 0 2 4P >0 0 5 )。Ⅱ型 5α 还原酶在增生前列腺组织中的表达水平与腺体的体积及腺体所在的部位相关。结论　Ⅱ型 5α 还原酶在良性前列腺增生的发生、发展过程中起着重要的作用 ,而Ⅰ型还原酶在良性增生组织中表达的意义需要进一步研究。Objective To study the expression levels and cell-specific expression patterns of steroid 5α-reductase type 1 and type 2 isoenzymes in human benign prostatic hyperplasia (BPH) tissues. Methods Immunohistochemistry and RT-PCR were used to investigate qualitatively and semi-quantitatively the expression of 5α- reductases type 1 and type 2 in 15 specimens of normal prostate tissues from dead kidney donor or patients undergoing cystectomy and 25 BPH tissues obtained from suprapubie enucleation. Results Both steroid 5α-reductase type 1 mRNA and protein and 5α-reductase type 2 mRNA and protein were present in the normal and BPH tissues, especially in the glandular epithelial cells and mainly in the cytoplasm. In the BPH group the expression of type 2 reductase in the periurethral tissues was significant higher than that in the subcapsular tissues (1.50±0.42 vs. 1.07±0.35, P <0.01), however, there was no significant difference in the expression of type 1 reductase between these 2 zones ( P >0.05). The expression level of 5α-reductase type 2 mRNA in BPH tissues was significantly higher than that in the normal specimens (e.g. in periurethral zone 1.50±0.42 vs. 0.98±0.32, P <0.01), but there was no statistically significant difference in the expression of 5α-reductase type 1 mRNA between these 2 groups (e.g. in periurethral zone 0.62±0.31 vs 0.51±0.24, P >0.05). In BPH tissues, the expression of type 2 mRNA was significantly higher in the larger prostate tissues (≥40 g) than in the smaller ones (e.g. in periurethral zone 1.58±0.47 vs. 1.14±0.46, P <0.05), however, there was no significant difference in the expression of 5α-reductase type 1 mRNA between the BPH tissues with different volumes. Conclusion Type 2 5α-reductase plays an important role in the pathogenesis and maintenance of BPH. The role of type 1 expression in the pathogenesis of BPH needs further research.

关 键 词：5Α-还原酶 良性增生前列腺组织 表达 免疫组织化学 前列腺增生 

分 类 号：R697.3[医药卫生—泌尿科学]