Effects of pH on structure andfunction of single living erythrocyte  

作　　者：YAOChengcan HUANGYaoxiong LIXiaokun RUANPing 

机构地区：[1]InstituteofBiomedicalEngineeringofJiNanUniversity,Guangzhou510630,China [2]BiopharmaceuticalR&DCenterofJinanUniversity,Guangzhou510630,China [3]DepartmentofPhysicsofGuilinMedicalCollege,Guilin541004,China

出　　处：《Chinese Science Bulletin》2003年第13期1342-1346,共5页

基　　金：supported partly by the National Natural Science Foundation of China(Grant No.30227001).

摘　　要：The spontaneous changes in cell shape and size, the membrane bending elastic modulus, and the structureand functions of intracellular hemoglobin of single humanliving erythrocyte incubated in different suspension pH were studied. The non-invasive, in situ, real time measurements on the parameters at the three levels of intracellular molecule, membrane and cell morphology were performedsimultaneously by using a fast micro-image analyzingtechnology and a multi-channel micro-spectrophotometer system. The result shows that the cell shape and size, themechanical property of the cell membrane, and the structure and function of intracellular hemoglobin are correlated and affected by each other with pH.The spontaneous changes in cell shape and size, the membrane bending elastic modulus, and the structureand functions of intracellular hemoglobin of single humanliving erythrocyte incubated in different suspension pH were studied. The non-invasive, in situ, real time measurements on the parameters at the three levels of intracellular molecule, membrane and cell morphology were performedsimultaneously by using a fast micro-image analyzingtechnology and a multi-channel micro-spectrophotometer system. The result shows that the cell shape and size, themechanical property of the cell membrane, and the structure and function of intracellular hemoglobin are correlated and affected by each other with pH.

关 键 词：PH值 红血细胞 血红蛋白 形态学 弹性模数 

分 类 号：R331.141[医药卫生—人体生理学]