Analysis of cDNA sequence,protein structure and expression of parotid secretory protein in pig  

作　　者：YIN Haifang, FAN Baoliang, ZHAO Zhihui, LIU Zhaoliang, FEI Jing & LI Ning State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100094, China Correspondence should be addressed to Li Ning (e-mail: ninglbau@ public3.bta.net.cn) 

出　　处：《Chinese Science Bulletin》2003年第13期1358-1363,共6页

基　　金：supported by the National Major Basic Research Development Program(Grant No.G20000161);Beijing Natural Science Foundation(Grant No.5030001).

摘　　要：Parotid secretory protein (PSP) secreted abundantly in saliva, whose function is related with the anti-bacterial effect. The PSP cDNA has been isolated from pig parotid glands by 3′ and 5′ rapid amplification of cDNA end (RACE), based on the conserved signal peptide region among the known mammalian PSP. The result of homologous comparison shows that pig PSP and human PSP shares the high identity at the level of the primary, secondary and tertiary protein structure. A search for functionally significant protein motifs revealed a unique amino acid sequence pattern consisting of the residues Leu-X(6)-Leu-X(6)-Leu- X(7)-Leu-X(6)-Leu-X(6)-Leu near the amino-terminal portion of the protein, which is important to its function. RT-PCR, Dot blot and Northern blot analysis demonstrated that PSP was strongly expressed in parotid glands, but not in other tissues.Parotid secretory protein (PSP) secreted abundantly in saliva, whose function is related with the anti-bacterial effect. The PSP cDNA has been isolated from pig parotid glands by 3′ and 5′ rapid amplification of cDNA end (RACE), based on the conserved signal peptide region among the known mammalian PSP. The result of homologous comparison shows that pig PSP and human PSP shares the high identity at the level of the primary, secondary and tertiary protein structure. A search for functionally significant protein motifs revealed a unique amino acid sequence pattern consisting of the residues Leu-X(6)-Leu-X(6)-Leu- X(7)-Leu-X(6)-Leu-X(6)-Leu near the amino-terminal portion of the protein, which is important to its function. RT-PCR, Dot blot and Northern blot analysis demonstrated that PSP was strongly expressed in parotid glands, but not in other tissues.

关 键 词：基因序列 腮腺分泌蛋白 猪 PSP 唾腺 

分 类 号：Q523[生物学—生物化学]