集胞蓝藻PCC6803含疏水亚基的NAD(P)H脱氢酶亚复合体的分离  

Separation of Hydrophobic NAD(P)H Dehydrogenase Subcomplexes from Cyanobacterium Synechocystis PCC6803

作　　者：邓勇[1] 叶济宇[1] 米华玲[1] 沈允钢[1] 

机构地区：[1]中国科学院上海生命科学研究院植物生理生态研究所,植物分子遗传国家重点实验室,上海200032

出　　处：《生物化学与生物物理学报》2003年第8期723-727,共5页

基　　金：国家重点基础研究发展规划项目 (973计划)(No .G19980 10 10 0);国家自然科学基金项目 (No.3 0 2 70 12 3)资助~~

摘　　要：利用离子交换与凝胶过滤层析 ,从n dodecylβ D maltoside(DM)处理的集胞蓝藻SynechocystisPCC6 80 3细胞粗提液中 ,首次分离到两个包含NDH疏水亚基NdhA的亚复合体。酶活性分析表明 ,分离到的NDH亚复合体具有NADPH 氮蓝四唑 (NBT)氧化还原酶活性 ,以NADPH为电子供体可以还原铁氰化钾、二溴百里香醌 (DBMIB)、二氯酚靛酚 (DCPIP)、duroquinone以及UQ 0等质醌类电子受体。Many efforts have been paid to the separation of an integrated NA(D)PH dehydrogenase (NDH) complex. Several hydrophilic subcomplexes of NDH have been purified from the cyanobacterium Synechocystis PCC6803. However, no hydrophobic NDH subcomplex has ever been separated from cyanobacteria yet. In this paper, two NDH subcomplexes were separated from n dodecyl β D maltoside(DM) treated whole cell extracts of Synechocystis PCC6803 by anion exchange chromatography and gel filtration. Both subcomplexes contained the hydrophobic subunit NdhA, suggesting that they were hydrophobic NDH subcomplexes. Of the two subcomplexes, only one subcomplex contained NdhH. These subcomplexes showed NADPH nitroblue tetrazolium (NBT) oxidoreductase activity and could specifically oxidize NADPH when several quinone analogues were used as electron acceptors, such as ferricyanide, 2,5 dibromo 3 methyl 6 isopropyl p benzoquinone (DBMIB), 2,6 dichlorophenol indophenol (DCPIP), duroquinone, ubiquinone 0 (UQ 0), etc.

关 键 词：集胞蓝藻 NAD(P)H脱氢酶 疏水亚基 类囊体膜 

分 类 号：Q943[生物学—植物学]