AFM对人乳腺癌细胞外纤连蛋白原纤维的形态学观察  

Morphological Observation on Fibronectin Fibrils Surrounding Human Breast Carcinoma Cells by Atomic Force Microscopy

作　　者：陈勇[1] 蔡继业[1] 

机构地区：[1]暨南大学生命科学与技术学院

出　　处：《生物化学与生物物理学报》2003年第8期752-755,共4页

基　　金：国家重点基础研究发展规划项目(973计划)(No .2 0 0 1CB5 10 10 1);国家自然科学基金重点资助项目(No .3 0 2 3 0 3 5 0 );国家自然科学基金资助项目(No .60 2 780 14 );教育部科技重点项目(No.0 0 12 4)~~

摘　　要：探讨原子力显微镜在研究细胞和细胞外基质间的相互作用及细胞外基质的功能等方面的应用前景。应用原子力显微镜观察培养的人乳腺癌MCF 7 R细胞分泌的纤连蛋白原纤维的分布和排列规律 ,并与其他常规观察技术进行比较。应用原子力显微镜获得了多个乳腺癌细胞和细胞外纤连蛋白原纤维的整体和局部形貌图像 ,发现这些原纤维的分布和排列方式非常有规律 ,而且这些规律与其功能相适应。由于样品制备简单和分辨率较高等优点 。This report aimed at investigation on the application of atomic force microscope (AFM) in research on interaction between cells and extracellular matrix, and function of extracellular matrix. Distribution and array of fibronectin fibrils surrounding human breast carcinoma MCF 7 R cells were observed by atomic force microscope, and comparison of AFM with other traditional techniques was performed. Whole and local AFM morphological images of several human breast carcinoma MCF 7 R cells and fibronectin fibrils around cells were obtained by AFM. Distribution and array of fibronectin fibrils were found to be very regular, and the regulated array coordinated with functions of fibronectin fibrils very well. Owing to advantages of simple sample preparation and very high resolution, AFM is an ideal tool to in situ observation of extracellular matrix.

关 键 词：原子力显微镜 人乳腺癌细胞 纤连蛋白 原纤维 

分 类 号：Q51[生物学—生物化学]