快速检测猪囊虫抗体的金免疫层析法的建立及应用  

Development of Gold immunochromatographic assay(GICA)for detecting the antibodies against Taenia Solium cysticercosis

作　　者：唐雨德[1] 周东明[1] 陆承平[2] 

机构地区：[1]南京军区军事医学研究所,南京210002 [2]南京农业大学动物医学院

出　　处：《中国人兽共患病杂志》2003年第5期77-79,共3页Chinese Journal of Zoonoses

摘　　要：目的　研制一种简便快速检测囊虫抗体的新方法 ,提供基层单位使用。方法　应用胶体金和免疫层析合成技术进行研究 ,建立检测囊虫抗体的金免疫层析法 ;以ELISA为对照 ,观察GICA法敏感性、特异性和稳定性。结果　每ml胶体金中兔抗猪IgG最适标记量为 30 8μg ,最佳抗原包被浓度为 1mg/ml。建立的GICA法检测囊虫病猪血清均为阳性 ,正常猪及其他疾病猪血清 ,结果均为阴性 ;整个实验仅一步操作 ,检测时间 5～ 15min。同ELISA法比较 ,两种方法检测囊虫抗体的相符率达 96 8% (P >0 0 5 )。GICA试纸条分别在 4℃和室温下保存 10 5d及 37℃保存 4 5d ,检测结果均未发现改变。结论　检测囊虫抗体的GICA法简便、快速、敏感、特异、稳定。Aim To develop a simple,fast,easy-to-do and new detecting method of the Abs against Taenia Solium cysticercosis for country veterinarian.Methods Gold immunochromatographic assay(GICA)was established by combining colloidal gold technique with immunochromatographic assay.Compared with enzyme-linked immunoabsordent assay(ELISA),its sensitivity,specificity and stability were evaluated.Results The optimum marked amount of Ab of one-milliliter colloidal gold was 30.8μg,the optimum concentration of the coating antigen was 1mg/ml.The Concordance was 96.8%(P>0.05)between the developed GICA and ELISA for detecting the antibodies against swine cysticerci.The results were negative for detecting the sera of normal pigs and other disease pigs.The whole detecting time was 5 to 15min.GICA strips coated with antigen and antibody can be stored at 4℃,room temperature for 105d and 37℃ for 45 d,repectively.Conclusion GICA detecting the antibodies against swine cysticerci is convenient,rapid,sensitiv,specific and stabile.

关 键 词：快速检测 猪囊虫 抗体 金免疫层析法 血清 

分 类 号：R446.6[医药卫生—诊断学] R383[医药卫生—临床医学]