当归多糖对人粒单系祖细胞和粒系白血病细胞株增殖分化的实验研究  

Experimental study of the effect of Angelica polysaccharide on proliferation and differentiation of human CFU-GM and HL-60 cells

作　　者：王莎莉[1] 王亚平[2] 戴勤[3] 姜蓉[2] 

机构地区：[1]重庆医科大学生理学教研室,重庆400016 [2]重庆医科大学组织胚胎学教研室,重庆400016 [3]重庆医科大学临床学院血液内科,重庆400016

出　　处：《重庆医科大学学报》2003年第3期257-260,共4页Journal of Chongqing Medical University

基　　金：国家自然科学基金资助课题 ( 3 9870 912)

摘　　要：目的 :研究当归多糖 (APS)对人粒单系造血祖细胞 (CFU -GM )和急性早幼粒白血病细胞株 (HL - 6 0 )增殖分化的影响。方法 :采用造血细胞体外培养 ,形态学观察 ,流式细胞术和免疫细胞化学等实验血液学技术。结果 :APS对正常CFU -GM的体外增殖有显著刺激作用 ;而对HL - 6 0细胞的增殖却有明显的抑制作用 ;APS可能通过阻止HL - 6 0细胞从静止期进入增殖周期 ,抑制DNA的合成等途径来抑制细胞增殖 ;APS可能诱导HL - 6 0细胞发生凋亡。结论 :APS有可能成为既能促进正常造血 ,又能抑制白血病等肿瘤细胞增殖 ,并诱导其凋亡或分化的天然诱导剂。Objective:To study modern biological mechanism for hematonic and antitumor of Angelica Sinensis (oliv) Diels.Methods:By using the techniques of hematopoietic cells culture in vitro,morphological observation,flow cytometry and immunocytochemistry,the effect of Angelica polysaccharide (APS) on proliferation and differentiation of human CFU-GM and promyelocytic leukemia cell line (HL-60) was studied.Results:APS could markedly promote the colony formation of CFU-GM and could significantly inhibit the proliferation of HL-60 in vitro.The inhibitory effect is concerned with the concentration of APS.APS may prevent HL-60 from the Go phase entering the S/G2+M phase,thus prohibit the synthesis of DNA.APS may also promote HL-60 entering the process of apoptosis.Conclusion:APS may not only promote normal hematopoiesis,but also inhibit the proliferation of leukemia cell and it can be a natural inducer for therapy of malignant tumor.

关 键 词：当归多糖 粒单系祖细胞 粒系白血病细胞株 人 

分 类 号：R331.1[医药卫生—人体生理学]