弹烧复合伤后海水浸泡对血管内皮细胞影响的实验研究  

An experimental investigation of the effect of seawater immersion on endothelial cells in dogs sustained by burn-firearm combined injury

作　　者：闫红[1] 赖西南 葛衡江[1] 

机构地区：[1]第三军医大学附属大坪医院野战外科研究所麻醉科,重庆400042 [2]第三军医大学附属大坪医院野战外科研究所第六研究室,重庆400042

出　　处：《第三军医大学学报》2003年第7期611-613,共3页Journal of Third Military Medical University

基　　金：全军"十五"医学科研指令性课题 ( 0 1L0 11)

摘　　要：目的　探讨弹烧复合伤合并海水浸泡对血管内皮细胞损伤的原因和机制 ,为海水浸泡早期救治提供一定的理论依据。方法　建立弹烧复合伤的犬实验模型 ,致伤后将犬随机分为非浸泡组和浸泡组 ,将浸泡组犬在海水中浸泡 4h后捞出 ,然后于伤后 4、7、10、2 0、2 8h分别取血检测循环内皮细胞 (CEC)以及血管性假血友病因子 (vWF)的变化。非浸泡组除不浸泡海水外 ,余检测时相及指标同浸泡组。结果　非浸泡组于致伤后 4h和 7hCEC、vWF升高 (P <0 .0 5 ) ,而浸泡组于伤后 4、7、10、2 0、2 8hCEC和vWF均持续增高 (P <0 .0 5 ,P <0 .0 1)。组间比较 :CEC从伤后 4h至伤后 2 8h ,浸泡组与非浸泡组相比有显著差异 (P <0 .0 5 ,P <0 .0 1) ,vWF于伤后 7h至伤后 2 8h浸泡组与非浸泡组相比有显著差异 (P <0 .0 5 ,P<0 .0 1)。结论　弹烧复合伤合并海水浸泡伤后可引起全身EC急性损伤 ,并且这种损伤比单纯弹烧复合伤 (不浸泡 )更严重 ,更持久。Objective To study the influence and mechanism of seawater immersion on endothelial cell injury sustained by burn firearm combined injury to improve the early therapeutic efficacy. Methods The dogs with burn firearm combined injury were randomly divided into two groups: immersion group and control group. In immersion group, the dogs were immersed in seawater for 4 hours, then taken out from seawater. Blood samples were collected from central vein at 4 h, 7 h, 10 h, 20 h and 28 h following wound for the detection of changes of the circulating endothelial cells (CEC) and von Willebrand Factor (vWF). The same procedures except immersion were performed in the control group. Results The levels of CEC and vWF elevated at 4 h and 7 h following wound in control group( P <0.05), but the levels of CEC and vWF significantly elevated at 4 h, 7 h, 10 h, 20 h and 28 h in immersion group( P <0.05 or P <0.01). There was significant difference between the immersion group and the control group( P <0.05 or P <0.01). Conclusion Seawater immersion after burn firearm combined injury can cause extensive acute damage to endothelial cells. The damage of immersion and burn firearm combined injury is more severe and longer than that of simple burn firearm combined injury.

关 键 词：海水浸泡 弹烧复合伤 犬 内皮细胞 

分 类 号：R322.12[医药卫生—人体解剖和组织胚胎学] R644[医药卫生—基础医学]