LPS对血管内皮细胞的直接损伤作用的初步研究  

Direct effects of LPS on the injury to vascular endothelial cells

作　　者：罗向东[1] 石富胜[1] 王晓军[1] 杨宗诚[1] 黎鳌[1] 

机构地区：[1]第三军医大学附属西南医院全军烧伤研究所

出　　处：《第三军医大学学报》2003年第18期1609-1612,共4页Journal of Third Military Medical University

摘　　要：目的　探讨烧伤后早期内毒素对血管内皮细胞 (Vasculancndothelialcells ,VEC)的直接损伤作用。方法　采用RF 6A13 5猴血管内皮细胞株和ECV 3 0 4人脐静脉内皮细胞株为模型 ,将细胞分为正常对照组和细菌脂多糖 (Lipopolysaco haride ,LPS)浓度梯度组。观察不同时相点LPS对内皮细胞形态、脱氢酶活性的影响、VEGF表达、VE cadherine的表达和LPS与VEC结合能力及对VEC中MAPK信号传导系统磷酸化的影响。结果　LPS对VEC的形态影响明显。 2 .0 μg mlLPS可明显激活内皮细胞的线粒体脱氢酶活性 ,并有时间依赖性 ;刺激内皮细胞 2～ 12h ,可诱导VEGF的表达 ;刺激内皮细胞 6～ 48h ,VE cadherine表达进行性下降。激光共聚焦显微镜和流式细胞仪分析显示LPS能与内皮细胞结合。免疫细胞化学和Westernblotting分析显示LPS可诱导ERK1 ERK2和P3 8MAPK磷酸化。结论　LPS能与VEC结合并损伤VEC的功能和形态。LPS对VEC的作用还伴有MAPK系统信号ERK1 ERK2和P3 8的磷酸化 。Objective To explore the direct effects of lipopolysaccharide (LPS) on the injury to vascular endothelial cells(VECs) at early postburn stage. Methods RF/6A135 and ECV 304 cell lines were employed as the model and were divided into control group and groups treated with LPS at different concentrations. The effects of LPS on the VEC morphological changes, the dyhydrogenase activities, the VEGF and VE cadherine expressions, the binding capacity of LPS to VEC and the phosphorylation of MAPK signal pathway at different time points were observed. Results The changes in the VEC morphology was obvious after LPS stimulation. The activity of mitochondrial dyhydrogenase could be activated dramatically by LPS at the concentration of 2.0 μg/ml in a time dependent manner. The expression of VEGF could be induced during 2 12 h after LPS stimulation but the expression of VE cadherine decreased progressively during 6 48 h after LPS stimulation. Analysis by laser confocal microscopy and flow cytometry revealed that LPS could bind to VEC. Immunocytometric and Western blot analysis revealed that the phosphorylation of ERK1/ERK2 and P38 MAPK could be induced by LPS. Conclusion LPS can bind to VEC. The morphology and function of VEC can be damaged by LPS with phosphorylation of ERK1/ERK2 and P38 molecules, suggesting that LPS can induce the trans membrane signal transduction of VEC.

关 键 词：LPS 血管内皮细胞 损伤 

分 类 号：R322.12[医药卫生—人体解剖和组织胚胎学] R392.11[医药卫生—基础医学]