烫伤诱导大鼠心肌细胞缺氧诱导因子-1α定位表达的研究  

Hypoxia inducible factor-1α expression in myocardial tissues of rats following severe burn injury

作　　者：党永明[1] 黄跃生[1] 陈丽峰[2] 

机构地区：[1]第三军医大学附属西南医院全军烧伤研究所 [2]第三军医大学高原军事医学系病理生理学教研室,重庆400038

出　　处：《第三军医大学学报》2003年第18期1602-1605,共4页Journal of Third Military Medical University

基　　金：国家重点基础研究发展规划资助项目 ("973"项目 ) (G19990 54 2 0 2 ) ;国家杰出青年科学基金资助项目 ( 30 12 50 4 0 ) ;全军"十五"指令性课题 ( 0 1L0 6 6 ) ;高等学校骨干教师资助计划项目 ( 2 0 0 0 ) ;军队首批临床高新技术重大项目 ( 2 0 0 2 )

摘　　要：目的　探讨 40 %Ⅲ度烫伤大鼠早期心肌组织中缺氧诱导因子 1α(HIF 1α)的蛋白表达的变化规律及其定位分布意义。方法　采用 40 %TBSAⅢ度烫伤大鼠模型 ,蛋白免疫印迹法 (Western blot)测定HIF 1α蛋白水平表达 ,免疫组化法定位显示HIF 1α表达。结果　烫伤后大鼠心肌组织内HIF 1α蛋白表达量明显升高。左、右心室HIF 1α蛋白表达不同 ,HIF 1α蛋白表达增加主要定位于细胞核。结论　严重烧伤可以诱导心肌组织HIF 1α蛋白表达增加。左心室对照组HIF 1蛋白含量明显高于右心室。HIF 1α蛋白表达增加主要分布于心肌细胞核内 ,诱导下游因子表达。Objective To investigate the changes and roles of hypoxia inducible factor 1α(HIF 1α) expression in myocardial tissues in rats following severe scald. Methods Male Wistar rats inflicted with 40% TBSA Ⅲ degree scald were used as animal models. HIF 1α protein in myocardial tissues was detected by Western blot and immunohistochemical technique. Results HIF 1α protein expression in rat myocardial tissues increased significantly in the early stage following scald. The difference in HIF 1α level between the left and right ventricles was significant. The increased HIF 1α protein was mainly located in the nucleus. Conclusion HIF 1α protein expression in myocardial tissues of rats can be induced by severe scald and HIF 1α protein expression in the left ventricle is significantly higher than that in the right ventricle. The increased HIF 1α protein in the nucleus can induce downstream cytokine expression.

关 键 词：烧伤 心肌缺血 缺氧诱导因子—1α 免疫印迹 免疫组化 

分 类 号：R322.11[医药卫生—人体解剖和组织胚胎学] R394.3[医药卫生—基础医学]