HSP70在严重烧伤大鼠心肌中的表达及其保护作用的研究  

Expression of heat shock protein 70 in myocardium of severely burned rats and its protective effect

作　　者：袁志强[1] 彭毅志[1] 李晓鲁[1] 张家平[1] 黄跃生[1] 杨宗城[1] 

机构地区：[1]第三军医大学附属西南医院全军烧伤研究所

出　　处：《第三军医大学学报》2003年第18期1620-1622,共3页Journal of Third Military Medical University

基　　金：国家重点基础研究发展规划资助项目 ("973"项目 ) (G19990 54 2 0 2 ) ;国家杰出青年科学基金资助项目 ( 30 12 50 4 0 ) ;全军"十五"指令性课题 ( 0 1L0 6 6 ) ;教育部高等学校骨干教师资助计划项目 ( 2 0 0 0 ) ;军队首批临床高新技术重大项目 ( 2 0 0

摘　　要：目的　研究热休克蛋白 70 (HSP70 )在严重烧伤大鼠心肌中的表达变化规律 ,并观察诱导热休克反应对烧伤后心肌损害的保护作用。方法　 72只Wistar大鼠随机分为单纯烫伤组 (B组 )、亚砷酸钠预处理组 (SA组 ) ,于致伤前及3 0 %TBSAⅢ度烫伤后 3、6、12、2 4、48h检测血清心肌钙蛋白I含量变化 ,Westernblot检测心肌组织HSP70蛋白表达情况。结果　大鼠严重烧伤后 3h心肌组织HSP70蛋白表达显著升高 ,伤后 48h达高峰。大鼠烫伤后 3h血清心肌钙蛋白I(cT nI)含量即开始明显升高 ,伤后 12h达高峰。亚砷酸钠预处理可显著诱导机体的热休克反应 ,SA组伤后多数时相点血清cTnI含量显著低于B组 ,且波动幅度较小。结论　严重烧伤早期即可引起心肌组织HSP70蛋白表达显著增强 。Objective To study the expression of heat shock protein 70 (HSP70) in myocardium and the protective effect of heat shock response on myocardium of scalded rats. Methods A total of 72 Wistar rats were randomly divided into 2 groups: simple burn group (B group) and sodium arsenite pretreatment group (SA group). Changes of serum cardiac troponin I(cTnI) content were determined at 0, 3, 6, 12, 24 and 48 h after 30% full thickness scald injury. Samples of myocardium were taken for the determination of HSP70 expression by Western blot analysis. Results HSP70 expression in myocardium increased significantly at 3 h after scald injury. The level of HSP70 was highest at 48 h after scald. Serum cTnI content increased significantly at 3 h after scald injury and reached the peak at 12 h after scald injury. Heat shock response could be induced by sodium arsenite pretreatment. SA group showed a significantly lower serum cTnI content. Conclusion Severe burn injury causes a significantly increased expression of HSP70 in myocardium. HSP70 may have endogenous anti injury effect on early burn injury of the myocardium.

关 键 词：热休克蛋白 心肌 烧伤 

分 类 号：R377.11[医药卫生—病原生物学] R394.3[医药卫生—基础医学]