HSP70基因静脉注射对严重烧伤大鼠肠粘膜保护作用的实验研究  

Protective effect of HSP70 gene transfection on intestinal mucosa injury in rats following severe burn

作　　者：李晓鲁[1] 彭毅志[1] 袁志强[1] 黄跃生[1] 杨宗城[1] 

机构地区：[1]第三军医大学附属西南医院全军烧伤研究所

出　　处：《第三军医大学学报》2003年第18期1626-1628,共3页Journal of Third Military Medical University

基　　金：国家重点基础研究发展规划资助项目 ("973"项目 ) (G1990 54 2 0 2 ) ;国家杰出青年科学基金资助项目 ( 30 12 50 4 ) ;全军"十五"指令性课题 ( 0 1L0 6 6 ) ;教育部高等学校骨干教师资助计划项目 ( 2 0 0 0 ) ;军队临床高新技术重大项目 ( 2 0 0 2 )

摘　　要：目的　为严重烧伤后肠粘膜损伤的防治提供可行性的基因治疗途径。方法　大鼠静脉注射含HSP70基因的重组腺病毒载体 ,通过严重烧伤后肠粘膜病理形态学改变、损伤指数统计及血清中乳酸脱氢酶含量的变化观察基因转染后保护效应。结果　静脉转染HSP70基因的大鼠烧伤后肠粘膜组织病理改变较单纯烧伤组减轻 ,损伤指数明显降低 ,血清中乳酸脱氢酶含量明显下降。结论　大鼠静脉注射含HSP70基因的重组腺病毒载体可保护严重烧伤后肠粘膜的缺血缺氧性损伤。为严重烧伤早期缺血缺氧性损伤的防治提供可行性的基因治疗途径。Objective To investigate the effectiveness of heat shock protein 70(HSP70) gene transfection in intestinal mucosa against hypoxic/ischemic injury following severe burn. Methods The recombinant adenovirus Ad HSP70 containing HSP70 gene was transfected into the severely burned rats through venal injection. The protective effect after gene transfection was observed by determination of the morphological changes of intestinal mucosa, injury indices and serum lactate dehydrogenase(LDH) following severe burn. Results Alleviated injury and significantly decreased injury indices and serum LDH were found in the transfected rats. Conclusion The Ad HSP70 transfection in rats can successfully induce HSP70 overexpression in intestinal mucosa and protect the rats against the hypoxic/ischemic injury in intestinal mucosa after severe burn.

关 键 词：烧伤 缺血缺氧 热休克蛋白质类70 腺病毒 

分 类 号：R452[医药卫生—治疗学] R459.9[医药卫生—临床医学]