Ndrg2基因及NDRG2蛋白的生物信息学分析  

Bioinformatic analysis of ndrg2 and NDRG2 protein

作　　者：张文红[1] 刘新平[1] 王琰[2] 韩月恒[1] 药立波[1] 

机构地区：[1]第四军医大学基础部生物化学与分子生物学教研室,陕西西安710033 [2]第四军医大学科研部全军基因诊断技术应用研究所,陕西西安710033

出　　处：《第四军医大学学报》2003年第17期1537-1539,共3页Journal of the Fourth Military Medical University

基　　金：国家自然科学基金 (30 0 70 773)

摘　　要：目的 :对ndrg2 (n mycdownsreamregulatorgene 2 )基因调控区和NDRG2蛋白序列进行生物信息学分析 ,从结构上预测其功能 .方法 :运用internet网络上的数据库及程序 ,对ndrg2基因的调控区和NDRG2蛋白的二级结构进行生物信息学分析 .结果 :ndrg2基因调控区存在多种转录因子结合位点 ,功能主要涉及组织特异性表达调控 ,细胞生长发育相关基因和应激反应基因的表达调控等 ;NDRG2蛋白的结构属于α/β水解酶类折叠 ,其分类预测表明与细菌环氧化物水解酶的二级结构极为相似 .结论 :生物信息学分析提示NDRG2蛋白具有一定的酶活性 ,可能参与细胞抗氧化应激反应 ,内外源有毒物质的代谢 。AIM: To obtain some functional clues from the structures of ndrg 2 gene and NDRG2 protein. METHODS: Bioinformatic analysis was performed on the upstream regulation region of ndrg 2 gene and the second structure of NDRG2 protein. RESULTS: Several binding sequences of some different transcription factors were found and these factors were involved in the regulation of tissue specific gene expression, the expression of genes related to growth and early development of cells and the expression of genes under some stimulated conditions. The results of protein fold classification showed that NDRG2 belonged to alpha/beta hydrolase fold family and high similarity was found between NDRG2 and epoxide hydrolase of bacteria. CONCLUSION : NDRG2 protein may be involved in resisting the oxidative stress, maintaining the balance of cell redox potential and regulating the metabolism process of xenobiotics or intracellular toxic molecules, which is very useful in further exploring the precise functions of NDRG2 protein.

关 键 词：NDRG2基因 NDRG2蛋白 生物信息学分析 α/β水解酶 

分 类 号：R61[医药卫生—外科学]