中国江、浙地区栽培大麦遗传资源的RAPD研究  

Genetic Variation Analysis by RAPD of Some Barley Cultivars in China

作　　者：施永泰[1] 边红武[1] 韩凝[1] 潘建伟[1] 童微星[1] 朱睦元[1] 

机构地区：[1]浙江大学生命科学学院

出　　处：《作物学报》2004年第3期258-265,共8页Acta Agronomica Sinica

基　　金：国家自然科学基金 ( 3 9770 42 0 ;3 0 10 0 115 );浙江省科技项目和国家 863项目资助

摘　　要：采用随机扩增多态性 (RAPD)标记 ,探讨了我国部分栽培大麦品种的遗传背景。结果表明 :2 0 0个随机引物中 ,有 30个引物扩增出的产物具有多态性 ,30个引物共扩增出 2 2 3条谱带 ,其中 130条谱带具有多态性 ,每个引物可扩增出2～ 9条多态性谱带 ,平均 4 .19条。 6 7个大麦品种平均表型多样性值 0 .36 9,裸麦的表型多样性高于皮麦的表型多样性 ,二棱皮麦表型多样性最低 ,六棱裸麦表型多样性最高。聚类分析表明 ,在遗传距离D值 0 .6水平上 6 7个大麦品种可聚成四簇 ,其中二棱皮麦单独聚成一簇 ,六棱裸麦单独聚成一亚簇 ,六棱皮麦、四棱皮麦、四棱裸麦混杂分布于其余各簇中 ,发现大麦品种根据其主要品种特性 ,表现出一定的聚集趋势 ,而抗逆性 (抗黄花叶病和耐湿性 )却表现出一定的分散性。Genetic variation is essential for genetic improvement and plant breeding. 200 arbitrary primers (10-mers) were used for the PCR amplification of random genetic DNA fragments in this study to estimate the genetic variation of 67 barley cultivars in China by RAPD, most of which were derived from the Zhejiang-Jiangsu planting zone. In total 223 bands or 130 polymorphic bands were amplified by 30 primers. Each primer could amplify 2 to 9 polymorphic bands, with an average of 4.19 bands. The value of phenotypic diversity in naked barley cultivars was higher than that of hulled barley cultivars. The lowest phenotypic diversity value was found in 2-rowed hulled barley cultivars. Cluster analysis showed that the 67 cultivars could be classified into 4 groups at the level of D 0.6, which evidently showed some variation of some important traits of barley.

关 键 词：江苏 浙江 大麦 栽培品种种 遗传多样性 RAPD 

分 类 号：S512.3[农业科学—作物学]