NPTⅡ基因和GUS基因在小麦遗传转化中的应用  

Application of NPT Ⅱ gene and GUS gene to wheat transformation

作　　者：张文蔚[1] 成卓敏[1] 

机构地区：[1]中国农业科学院植物保护研究所国家病虫害生物学重点实验室,北京100094

出　　处：《植物保护》2004年第1期48-50,共3页Plant Protection

摘　　要：对小麦遗传转化上常用的选择标记基因NPTⅡ的选择剂及GUS基因在幼胚愈伤组织中的转移进行了研究。结果表明用G418作为选择剂,浓度为20-30 mg／L较为合适。幼胚愈伤组织与农杆菌共培养3 d后,即可检测到GUS基因的瞬时表达。农杆菌LBA4404由500 mg／L羧卞青霉素抑制;EHA105、AGLI由500 mg／L头孢霉素抑制。而不能用卡那霉素。The selectable marker gene ( NPT Ⅱ ) that is usually used for wheat transformation was tested for the optimal selector. The results indicated that 20 - 30 mg/L G418 was suitable for selection of NPT Ⅱ gene. Transient expression of GUS gene was detected in the tissues after 3 days of co-culture with Agrobacterium tumefaciens. Agrobacterium strain LBA4404 was inhibited by 500 mg/L Carbenicillin; EHA105 and AGLI were inhibited by 500 mg/L Cefotaxime.

关 键 词：NPTⅡ基因 GUS基因 小麦 遗传转化 幼胚愈伤组织 农杆菌介导 

分 类 号：S512.1[农业科学—作物学]