结核分枝杆菌利福平耐药性的研究进展  

Evolution of the rifampin resistance in Mycobacterium tuberculosis

作　　者：曹立雪[1] 林艳丽[2] 吴雪琼[3] 

机构地区：[1]白求恩军医学院,石家庄050081 [2]军事医学科学院生物工程研究所,北京100071 [3]解放军第三〇九医院,北京100091

出　　处：《生物技术通讯》2004年第1期79-82,共4页Letters in Biotechnology

摘　　要：本文旨在阐明结核分枝杆菌耐利福平分离株rpoB基因突变的规律,以及rpoB基因突变与利福平最低抑菌浓度(MIC)的关系。结核分枝杆菌的rpoB基因突变是引起利福平耐药性的主要原因,耐利福平分离株的rpoB基因突变主要集中在507～533位密码子的81bp的区域内,约80%的菌株发生531位或526位密码子突变。不同类型rpoB基因突变的结核分枝杆菌对利福平的耐受性也不同,通常发生531位密码子突变的菌株的MIC≥64μg/mL。The paper was aimed at the regularity of rpoB gene mutations at the rifampin resistant Mycobacterium tubercu-losis and the relationship between rpoB gene mutations and rifampin minimal inhibitory concentrations(MIC).The mutations in rpoB gene were the main reasons of the rifampin resistance in Mycobacterium tuberculosis.The mutations were occurred at the81bp region from codon507to codon533.About80%rifampin-resistant Mycobacterium tuberculosis strains had rpoB gene mutations in codon531or in codon526.The strains with different genotypes of rpoB gene had different drug tolerance.In common,the MIC were above64μg/mL when the strains had rpoB gene mutations in codon531.

关 键 词：结核分枝杆菌 利福平 最低抑菌浓度 RPOB基因 耐药性 

分 类 号：R378.911[医药卫生—病原生物学]