SARS-CoV S蛋白基因的克隆及其与VSV-G融合表达载体的构建  

Cloning of the gene of SARS-CoV S protein and construction of the fusion expression vector of S protein with VSV-G

作　　者：冯彦斌[1] 善亚君[2] 苑晓玲[2] 杨振[2] 赵振虎[2] 王升启[2] 刘晓辉[1] 从玉文[2] 

机构地区：[1]山西大学生命科学与技术学院,太原030006 [2]军事医学科学院放射医学研究所,北京100850

出　　处：《生物技术通讯》2004年第1期9-12,共4页Letters in Biotechnology

摘　　要：为了解重症急性呼吸综合征冠状病毒(SARS-CoV)表面S蛋白的受体结合功能域及其在宿主细胞上的作用受体,应用PCR技术从SARS-CoVcDNA中克隆到S蛋白的全长基因,并构建了S蛋白与疱疹性口腔炎病毒胞膜蛋白(VSV-G)融合表达载体pVSV-G'-SG,进而为制备含有SARS-CoVS蛋白膜外区的逆转录病毒假毒粒奠定了实验基础。The tropism or host range could be altered by changing the viral envelope protein.In order to find the recep-tors in the host cells and invest the receptor binding domain of the severe acute respiratory syndrome coronavirus(SARS-CoV)spiking(S)protein,the full gene of S protein was cloned from the complete cDNA of SARS-CoV,and the fusion ex-pression vector pVSV-G'-SG was constructed with the extracellular domain of S protein and transmembrane region and cy-toplasmic tail of vesicular stomatitis virus(VSV)G glycoprotein by PCR method.This work is the first step to generate pseudotyped virus with S/VSV-G chimeric envelope.

关 键 词：SARS—COVS蛋白基因 克隆 VSV—G融合表达载体 构建 急性呼吸综合征冠状病毒 SARS 

分 类 号：Q786[生物学—分子生物学] R373.1[医药卫生—病原生物学]