难溶性药用纳米微粒的原子力显微检测  

Measuring indissoluble medical nanoparticles with atomic force microscope

作　　者：曲秋莲[1] 张英鸽[1] 孙岚[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850

出　　处：《生物技术通讯》2004年第1期45-47,共3页Letters in Biotechnology

基　　金：军队"十五"规划科研基金重点课题(01Z024)

摘　　要：建立用原子力显微镜(AFM)检测难溶性药用纳米微粒的方法。将纳米微粒配成一定浓度的悬浮液,以新鲜裂解的云母表面作为样品载体,在室温下用AFM的接触模式成像,用粒度分析软件进行粒度分析。以此方法对两种微粒(活性炭及氧化锌纳米微粒)进行了成像和测量,同时对两种样品进行透射电镜(TEM)观察。AFM检测结果显示,纳米活性炭微粒形态近似球形,表面较平滑,平均直径为(299±187)nm;纳米氧化锌微粒呈球形,平均直径(50±20)nm,与TEM检测结果具有较高一致性。该法简便可靠。To establish a method for measuring indissoluble medical nanoparticles with atomic force microscope(AFM).Nanoparticles are diluted in water to a certain concentration.Newly cleaved mica is used as carrier.To image the nanoparticles by AFM in contact mode.All measurements are carried out at room temperature.The activated carbon nanoparticles and the zincic oxide nanoparticles were imaged and measured by the method.AFM result showed that most of the activated carbon nanoparticles were nearly round.The average diameter of them was(299±187)nm.The average di-ameter of the zincic oxide nanoparticles was(50±20)nm.The result of AFM was coincident with that of TEM.The method of AFM for measurement of indissoluble nanoparticles is simple and reliable.

关 键 词：原子力显微技术 纳米微粒 活性炭 氧化锌 

分 类 号：Q6-33[生物学—生物物理学] Q66