中草药抗仔猪黄痢作用机理的探索  

Study on mechanism of action of Chinese herbal medicine on yellow scour of piglet

作　　者：刘玉芹[1] 张秀英[1] 李群道[1] 隗金玲 

机构地区：[1]东北农业大学动物医学院,黑龙江哈尔滨150030 [2]宾县畜牧局,黑龙江哈尔滨150030

出　　处：《东北农业大学学报》2004年第1期50-54,共5页Journal of Northeast Agricultural University

摘　　要：从中草药对大肠杆菌的体外抑菌活性及对热敏肠毒素 L T所致结扎肠袢液体蓄积的拮抗作用两个方面 ,初步探讨了大青叶、板蓝根、连翘、败酱草、盐酸黄连素 5味中草药及其复方配伍抗仔猪黄痢的作用机理。试验结果表明 ,板蓝根、连翘、败酱草等及其复方制剂对大肠杆菌 O1 49K88均显示出不同程度的体外抗菌活性 ;将上述中药提取液加入到细菌培养液中与大肠杆菌一起培养 ,用所得培养物 L T进行兔回肠结扎试验 ,均可显著或极显著地减少培养物上清液所致肠袢液体蓄积量 (P <0 .0 5 ,P <0 .0 1) ;将败酱草提取液同 L T一起注入结扎肠袢 ,可显著地减少肠袢液体蓄积量 (P <0 .0 5 )。由此可说明 ,抑制病原菌的繁殖、拮抗热敏肠毒素的致泻作用是中草药治疗仔猪黄痢的重要机理 ,该结果为临床应用中草药治疗仔猪黄痢病提供了重要理论依据。In this paper,the mechanism of action of the five Chinese herbal medicines,which are Isatis leaf,Isatis root,Forsythia fruit,Patrinia and Berberine hydrochloride,and their complex prescriptions on yellow scour of piglet,was preliminarily studied from two aspects which are the bacteriostatic activity in vitro on E.coli and the antagonism against liquid retention mediated by E.coli heat-labile enterotoxin (LT).The results showed that Isatis root,Forsythia fruit,Patrinia and their complex prescriptions all had more or less bacteriostasis on E.coli.O 149 K 88 Escherichia coli was cultured in bacterial culture medium to which the extracts of the Chinese herbal medicines were added,and the cultural product LT was used to do experiment in ligated rabbit ileum.They all significantly or high significantly reduced the liquid retention caused by cultural supernatant fluid in ligated loops(P<0 05,P<0 01).When the extracts of Patrinia and LT were directly injected into ligated loops together,Patrinia significantly reduced the liquid retention(P<0 05).The aforementioned results indicated that the bacteriostatic activity and the antagonism against diarrhea caused by enterotoxin LT were important mechanism of Chinese herbal medicines on the yellow scour of piglet.The results provided an important theoretical basis for clinically applying Chinese herbal medicines to the yellow scour of piglet.

关 键 词：中草药 仔猪 黄痢 作用机理 大肠杆菌 体外抑菌活性 抗热敏肠毒素 

分 类 号：S858.28[农业科学—临床兽医学] S853.74[农业科学—兽医学]