钙调素激酶在玉米体内的免疫组织化学定位  

Immunohistochemical Localization of Calmodulin Kinase in Maize

作　　者：杨万年[1] 梁述平[1] 吕应堂[1] 

机构地区：[1]武汉大学植物发育生物学教育部重点实验室

出　　处：《武汉植物学研究》2004年第1期33-38,共6页Journal of Wuhan Botanical Research

基　　金：国家自然科学基金资助项目(30170449)。

摘　　要：应用免疫组织化学定位方法研究了玉米体内钙调素激酶(CaMkinase,CaMK)的表达模式。结果表明CaMK广泛分布于玉米体内,但表达水平存在着明显时空差异。在营养器官中钙调素激酶主要分布于叶的维管束鞘细胞、侧根原基和根尖等部位,而其它部位没有检测到明显的分布。在生殖器官中,有大量钙调素激酶分布于幼胚及花药小孢子母细胞、四分体及绒毡层细胞中;在成熟胚囊的卵细胞、中央细胞以及二者的分界面上也有少量分布。这些结果为进一步探索钙调素激酶在植物体内的生理功能提供了重要线索。The expression patterns of a maize calmodulin kinase (CaM kinase,CaMK) by immunohistochemical localization were studied. The results indicated that while the CaMK was widely distributed in maize,from vegetative to reproductive organs,its expression was highly re-(gulated) spatially and temporally.CaMK was mainly accumulated in vascular bundle sheath cells of leaves and highly expressed in lateral root primordia and root tips.But the kinase was under detectable in other regions.In reproductive organs,much CaMK was found in young embryos and some anther cells such as microspore mother cells,tetrads and tapetum.In mature embryo sacs,significant amounts of CaM kinase was also observed in the egg cell,central cell and at the interface of these two cells.All these findings provided clues for biological functions of thes CaM kinase in maize.

关 键 词：钙调素激酶 免疫组织化学定位 胚 花药 玉米 

分 类 号：Q945.45[生物学—植物学]