水稻幼穗培养高效再生系统的建立  

Efficient Regeneration from In Vitro Culture of Young Panicles of Rice (Oryza sativa L.)

作　　者：王亚琴[1] 段中岗[1] 黄江康[2] 梁承邺[1] 

机构地区：[1]中国科学院华南植物研究所,广州510650 [2]广东省科技厅,广州510070

出　　处：《植物学通报》2004年第1期52-60,共9页Chinese Bulletin of Botany

基　　金：广东省自然科学基金资助项目(990770)。

摘　　要：本文测试了不同基因型幼穗取材时期、消毒方式、4℃下处理时间、愈伤组织诱导、分化及生根条件等对水稻幼穗培养再生成株的影响,实验结果表明,在优化条件下,籼稻的绿苗分化率在85%以上,成苗率115%左右;粳稻的绿苗分化率在90%以上,成苗率130%左右;粳型广亲和的绿苗分化率、成苗率分别达到85%和115%以上。由此建立了一套高效、可靠、重复性好的水稻组织培养再生系统,为水稻遗传转化的顺利进行奠定了基础。Factors affecting the regeneration of rice plants from young panicles , such as develop-mental stage of young panicles in different genotypes, sterilizing methods, duration of 4℃ treatment,induction, differentiation and rooting conditions, etc., were investigated. The results showed that theoptimal conditions for different genotypic rice were as following: after the best developmental stage( Ⅲ~Ⅳ of indica,Ⅰ~Ⅱ of japonica,Ⅴ of wide compatible variety, WCV) of young panicles wastreated at 4℃ within 5 days, the materials were sterilized with 0.5% potassium hypochlorite for 20minutes, cultivated in YS-Y medium about 20 days to induce the calli , then transferred to YS-YF、YS-F medium about 15 days to regenerate shoots and YS-SG medium for 1~2 weeks to induce the roots.Under the optimal conditions, the frequency of green shoot differentiation was a little more than 85%(indica), 90% (japonica), 85% (WCV), the frequency of root regenerated was about 115% (indica),130% (japonica), 115% (WCV), respectively. Therefore a high efficient regeneration system from invitro culture of young panicles of rice was established for genetic transformation of rice.

关 键 词：水稻 幼穗 组织培养 再生系统 

分 类 号：S511[农业科学—作物学]