悬铃木叶片DNA提取方法研究  

Study on methods for extraction of DNA from Platanus orientalis leaves

作　　者：范国强[1] 李锋[1] 赵振利[1] 蒋建平[1] 宁玉献[2] 

机构地区：[1]河南农业大学,河南郑州450002 [2]河南省科技情报研究所,河南郑州450003

出　　处：《河南农业大学学报》2004年第1期68-72,共5页Journal of Henan Agricultural University

基　　金：河南省杰出人才创新基金(0321001700);高校杰出科研人才创新工程资助项目(2002kycx).

摘　　要：以悬铃木组织培养苗叶片为材料,比较了提取叶片DNA的4种方法:SDS-Ⅰ,SDS-Ⅱ,CTAB-Ⅰ,CTAB-Ⅱ,并用紫外光谱吸收、凝胶电泳、限制性内切酶酶切、RAPD等方法进行鉴定.研究结果表明,4种方法提取的DNA的得率约为0 347～0 518μg·mg-1,分子量约为23kb,均可被酶切.此外,CTAB-Ⅰ和CTAB-Ⅱ提取的DNA可用于RAPD分析.综合考虑得率、纯度等因素,作者认为CTAB-Ⅱ法可作为悬铃木叶片DNA提取的最佳方法.This paper dealt with extraction of DNA from Platanus orientalis leaves with 4 methods:SDS-Ⅰ, SDS-Ⅱ, CTAB-Ⅰand CTAB-Ⅱ,and they were tested with ultraviolet spectrophotometer anaylis, agarose gel electrophoresis, restriction enzyme digestion and RAPD reaction respectively. The results indicated that 3.47～5.18(μg·mg^(-1)) DNA was obtained from the fresh leaves,their molecular weights were about 23 kb,and they were all suitable for restriction enzyme digestion. Moreover, DNA ebtained with CTAB methods might be used for RAPD analysis. All fectors considered,CTAB-Ⅱ was considered as one of the best methods for extraction of DNA from Platanus orientalis leaves.

关 键 词：悬铃木 DNA提取 限制性酶切 RAPD 

分 类 号：S792.37[农业科学—林木遗传育种] Q943[农业科学—林学]