同步辐射X荧光法测定电泳分离后蛋白条带内的锌  

Analysis of zinc in protein bands after electrophoresis separation by synchrotron radiation X-ray fluorescence

作　　者：高愈希[1] 丰伟悦[1] 李柏[1] 章佩群[1] 何伟[2] 黄宇营[2] 柴之芳[1] 

机构地区：[1]中国科学院高能物理研究所核分析技术重点实验室,北京100039 [2]中国科学院高能物理研究所同步辐射国家实验室,北京100039

出　　处：《核技术》2004年第3期165-168,共4页Nuclear Techniques

基　　金：国家自然科学基金 (No.20071032);中国科学院重大项目 ( KJCX2-N01 )资助课题

摘　　要：本文初步建立了一种基于电泳分离和同步辐射X荧光(SRXRF)分析技术的生物样品内微量元素的种态分析方法。用同步辐射X荧光法直接分析了人肝细胞胞质溶胶等电聚焦(IEF)分离后蛋白条带内的金属锌含量。结果表明:肝细胞胞质溶胶内主要有三个含锌条带,等电点(pI)分别为4.69、5.93、9.84。含锌量分别占总检出锌的33.08%、17.05%、26.54%。其中等电点为4.69的蛋白可能为金属硫蛋白。另外在等电点为6.60—9.30的范围内有若干含锌蛋白,共占总检出锌的23.33%。A procedure based on isoelectric focusing ( IEF ) separation and synchrotron radiation X-ray fluorescence ( SRXRF ) analysis for speciation of trace elements in biological samples was preliminarily established. The proteins in human liver cytosol were separated by IEF, and zinc amount in each protein band was measured by SRXRF. The results indicate that there were three main zinc-containing bands with isoelectric points ( pIs) of 4.69, 5.93, and 9.84 present in the sample, and the zinc percentages in the three bands to the total recovered zinc were 33.08%, 17.05%, and 26.54%, respectively. In addition, several zinc-containing proteins were present in the pI range of 6.6 ? 9.3, which amounted to 23.33 % of the recovered zinc.

关 键 词：形态分析 含锌蛋白 同步辐射X荧光 等电聚焦 人肝细胞胞质溶胶 

分 类 号：Q51[生物学—生物化学] Q503