植物中硝态氮、氨态氮、总氮测定方法的比较研究  

Study on the Method for the Determination of Nitric Nitrogen, Ammoniacal Nitrogen and Total Nitrogen in Plant

作　　者：吕伟仙[1] 葛滢[1] 吴建之[2] 常杰[1] 

机构地区：[1]浙江大学生命科学学院,浙江杭州310029 [2]浙江大学分析测试中心,浙江杭州310028

出　　处：《光谱学与光谱分析》2004年第2期204-206,共3页Spectroscopy and Spectral Analysis

基　　金：国家重点基础研究规划项目 (G2 0 0 0 0 4680 5 );国家自然科学基金项目 ( 3 9970 0 5 8)资助

摘　　要：植物样品经硫酸 +过氧化氢凯氏法消煮后 ,分别用直接吸光光度法、靛酚蓝比色法、过硫酸钾氧化吸光光度法获得硝态氮 (包括亚硝态氮 )、氨态氮、总氮的含量。通过样品测试数据的比较 ,证明分别测得的氨态氮、硝态氮含量之和与所测总氮结果一致。This paper reports the determination of three nitrogen states in plants: nitric nitrogen, ammoniacal nitrogen and total nitrogen. The plant sample was digested by the Kjeldathl method with H 2SO 4+H 2O 2. The solution of the sample was determined by three methods: absorption photometric method, indigotic colorimetry, and UV absorption photometric method after K 2S 2O 8 oxidation. By comparing the results, the relationship among the three N states is that the total N= NH + 4+NO - 3. Suitable methods were found for the determination of the elements, thus providing a scientific basis for the selection of suitable methods for chemical element analysis in plants.

关 键 词：植物 硝态氮 氨态氮 总氮 测定方法 含量测定 比较研究 凯氏法 硫酸 过氧化氢 

分 类 号：Q946[生物学—植物学]