巢蛋白和胰岛素在大鼠胰腺不同发育阶段的表达  

The Expression and Location of Nestin in Pancreas during Embryonic, Newborn and Adult Rats

作　　者：倪雪峰[1] 彭韬[2] 程志祥[1] 熊俊[1] 顾冬民[1] 徐桦[1] 德伟[1] 

机构地区：[1]南京医科大学生物化学和分子生物学系,江苏南京210029 [2]南京医科大学病理教研室,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2004年第2期97-99,F002,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省自然科学基金资助项目(BK2001119)

摘　　要：目的:探讨巢蛋白(nestin)在胚胎后期、新生期、成年3阶段的mRNA、蛋白水平表达的变化。方法:应用RT-PCR、免疫组化的方法进行半定量、定位分析。结果:在胚胎后期、新生期、成年3阶段Nestin在mRNA、蛋白水平均有表达,Nestin阳性细胞主要分布在胰岛中,腺泡周围的外分泌组织中有少量分布。结论:Nestin随大鼠胰腺的生长发育,核酸、蛋白表达下调,胰岛内Nestin阳性细胞百分数表达也下调。Objective:To study the expression of nestin and its mRNA in pancre as of different phases, including the embryonic, newborn and adult rats. Methods: The mRNA of nestin was detected by RT-PCR,the number of nestin-positive cells and the levels of nestin protein were accessed by the methods of immunohistoche mistry, and the results were analyzed by the statistical methods. Results:Nestin was expressed in E18.5,newborn and adult phases of rats. And nestin positive ce lls were mainly located in the islets and sparsely in the periacinar. Conclusion : The level of mRNA and protein of nestin in E18.5 was higher than that in new b orn and adult phase. The positive cells in islets in E18.5 was higher than adult phase. The nestin positive islet-derived cell in E18.5 may be a better resourc e for the research of stem cell than adult rat. [

关 键 词：巢蛋白 胰岛素 胰腺 RT-PCR 免疫组化 

分 类 号：R335.6[医药卫生—人体生理学] Q344[医药卫生—基础医学]