机械应力作用皮肤成纤维细胞增殖的相关研究  

A research on proliferation of skin fibroblast cultured with mechanical stress

作　　者：舒茂国[1] 郭树忠[1] 韩岩[1] 杨力[1] 张琳西[1] 贾平[1] 夏文森[1] 

机构地区：[1]第四军医大学西京医院整形外科中心,陕西西安710032

出　　处：《中国实用美容整形外科杂志》2004年第1期47-50,共4页

基　　金：陕西省自然科学基金资助 (2 0 0 1SM52 )

摘　　要：目的　研究机械应力作用皮肤成纤维细胞增殖效应。方法　自行设计一种新的机械牵拉细胞培养模型 ,原代培养皮肤成纤维细胞 ,取 3～ 5代的成纤维细胞种植在模型的弹性膜上 ,待细胞达到 75 %～ 80 %融合度时 ,实施 4 0 %延长度的机械牵拉 ,检测实验组及对照组 12、2 4、36、4 8、72h时间点的细胞数 ,利用MTT法检测不同时间点细胞的增殖变化 ,同时利用流式细胞仪检测不同时间点的细胞增殖周期。结果　采用 4 0 %延长度的机械牵拉可以明显导致细胞增殖 ,同时 ,代表细胞增殖特性的S期也随机械牵拉发生改变。结论　 4 0 %延长度的机械牵拉可以改变细胞的增殖周期 。Objective To study the expression of the proliferation of human skin fibroblasts treated with mechanical stress. Methods A new cell culture model was designed which can load mechanical strain. Primary cultured skin fibroblasts were obtained from full thickness skin residual in skin-graft surgery. The 3～5 pages fibroblasts were planted on elastic membranes of the model. After the 75%～80% confluence of the cell was reached, mechanical stretch was applied. The extend degree was 40% in the experiment. The sample were divided into the tested group and the control, counted the number of the cells individually 12、24、36、48、60、72?h after the begin of the mechanical stress, drew the proliferation curve and compared the cell proliferation between the tested group and the control in different time with MTT. The cell cycle was assessed with FACS. Results After stretching, we found the maximum percentage of S phase cell appeared 36h after the strain. The number of the cells of the test group was apparently increased compared with the number in control group. Conclusion Mechanical stretch with 40% extend degree and one time but continues can change the cell cycle and improve the cell proliferation.

关 键 词：机械应力 皮肤成纤维细胞增殖 机械牵拉 细胞培养 

分 类 号：R318.01[医药卫生—生物医学工程]