大鼠大脑皮层星形胶质细胞的传代培养  

The Secondary Culture of Astrocytes from Cerebral Cortex of Rats

作　　者：谢桂庭[1] 李回军[1] 骆建文[1] 康兆恒[1] 梁健勤[1] 田东萍[2] 

机构地区：[1]汕头大学医学院99级2班大学生科研小组,广东汕头515041 [2]汕头大学医学院病理学教研室

出　　处：《汕头大学医学院学报》2003年第3期162-163,F002,共3页Journal of Shantou University Medical College

摘　　要：目的 :获得纯度较高的星形胶质细胞材料用于进一步实验。方法 :采用大鼠神经胶质细胞的原代培养和传代培养方法。取生后 2 4h以内Wistar大鼠脑皮层 ,用机械法和胰蛋白酶消化法分散细胞 ,制成细胞悬液 ,接种于无底物黏附培养瓶中 ,置 37°C ,5 %CO2 培养箱中培养。培养液为DMEM F1 2 混合培养液。待细胞铺满瓶底后传代 ,并对传代细胞进行形态观察、吖啶橙荧光染色和星形胶质细胞的免疫细胞化学鉴定。结果 :通过传代得到了形态典型含量较高的星形胶质细胞。结论 :在无底物黏附培养的条件下 ,星形胶质细胞在体外能够得到良好的生长 。Objective:To purify astrocytes for further experiments.Methods:Primary culture and passage of astrocytes in vitro.The cerebral cortex was obtained from wistar rats born in 24 hours,and prepared cell suspension by digestion with the use of 0.25% trypsin and mechanical digestion,then transferred to culture flasks,and cultured(37°C,5% CO 2).The culture medium was DMEM/F12.The primary culture was passaged when it was confluent.Finally the secondary culture was observed,stained with acridine orange(AO)and HRP immunocytochemical staining by glial fribrillary acidic protein(GFAP).Results:By passage,the purified astrocytes with typical shapes were got.Conclusion:Astrocytes can grow well and be purified in vitro,without any stratum.

关 键 词：大鼠 大脑皮层 星形胶质细胞 细胞传代培养 胶质纤维酸性蛋白 免疫细胞 

分 类 号：R338.2[医药卫生—人体生理学] R329.28[医药卫生—基础医学]