uPA和uPA-R在人胚胎角膜碱烧伤后修复过程中作用的体外研究  

Roles of uPA and uPA-R in the course of repair after human embryo corneal alkali burn in vitro

作　　者：严军[1] 杨恬[1] 李国平[1] 高强国[1] 杨进[1] 

机构地区：[1]第三军医大学基础医学部细胞生物学教研室,重庆400038

出　　处：《第三军医大学学报》2003年第15期1318-1321,共4页Journal of Third Military Medical University

基　　金：国家重点基础研究发展规划资助项目 ("973"项目 ) (G19990 54 2 0 4 )

摘　　要：目的　研究体外条件下人胚胎角膜碱烧伤后修复过程中尿激酶型纤溶酶原激活剂 (UrokinasetypePA ,uPA)和尿激酶型纤溶酶原激活剂受体 (uPA receptor ,uPA R)的作用。方法　在建立体外碱烧伤模型的基础上 ,采用细胞培养和形态学观察、ICC法以及图像分析等技术。结果　角膜缘和中央角膜源性的离体细胞的生长情况无明显差异 ,几乎所有细胞均表达AE1 AE3。碱烧伤后uPA和uPA R的表达在碱烧伤后 2 4h左右达到峰值 ,其中uPA的表达部位多位于胞浆 ,uPA R则相对较多位于胞膜。结论　组织块法培养可以获得纯度较高的角膜上皮细胞 ,胚胎组织的发育和细胞活力与成体角膜之间可能存在着差异 ,uPA和uPA R的作用在上皮组织中也可能存在着较强的共性。Objective To explore the roles of urokinase type PA (uPA) and uPA receptor (uPA R) in the course of repair after human embryo corneal alkali burn in vitro . Methods After the alkali burn model in vitro was established successfully, some techniques such as the observation of cell culture and morphology, ICC and image analysis were used. Results No significant difference was found between the cells from corneal limbus and central cornea and almost all of them expressed AE1/AE3. The expressions of uPA and uPA R increased to the maximum at about 24 h after alkali burn. uPA expression distributed mainly in the cytoplasm but uPA R expression distributed mainly in the cell membrane. Conclusion The corneal epithelial cells of comparatively high purity can be acquired by tissue culture. There probably exists difference in development and vitality between embryo and adult cornea. There might be commonness of the roles of uPA and uPA R in epithelial tissue.

关 键 词：尿激酶型纤溶酶原激活剂 和尿激酶型纤溶酶原激活剂受体 人胚胎角膜 碱烧伤 修复 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学] R322.91[医药卫生—基础医学]